Wang Biao, Xu Lingyan, Ge Yugang, Cai Xiaomin, Li Qiang, Yu Zhengyi, Wang Jiawei, Wang Ying, Lu Chao, Wang Danping, Wang Yuanyuan, Chen Xiaofeng, Gu Yanhong
Clin Lab. 2019 Jan 1;65(1). doi: 10.7754/Clin.Lab.2018.180541.
Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3) has been proven to be involved in various human cancers; however, the function of PLOD3 in gastric cancer (GC) remains unclear. In this study, the role of PLOD3 in GC was evaluated.
The expression of PLOD3 in GC tissues and normal tissues was predicted by The Cancer Genome Atlas (TCGA). The kmplot online tool was performed to evaluate the impact of PLOD3 expression on GC patients' survival. Real-time PCR was conducted to verify PLOD3 expression in our own clinical samples and GC cells. The Cell Counting Kit-8 and the colony formation assay were used to detect GC cell proliferation ability.
PLOD3 was upregulated in human GC tissues (compared to adjacent normal tissues, p < 0.001) and GC cells. High expression of PLOD3 was significantly correlated with larger tumor size (p = 0.007) and poor prognosis. Inhibition of PLOD3 could suppress cell proliferation in GC.
These results revealed that PLOD3 may promote the progression of GC.
原胶原蛋白赖氨酸2-氧代戊二酸5-双加氧酶3（PLOD3）已被证实参与多种人类癌症；然而，PLOD3在胃癌（GC）中的功能仍不清楚。在本研究中，评估了PLOD3在GC中的作用。
通过癌症基因组图谱（TCGA）预测PLOD3在GC组织和正常组织中的表达。使用kmplot在线工具评估PLOD3表达对GC患者生存的影响。进行实时PCR以验证PLOD3在我们自己的临床样本和GC细胞中的表达。使用细胞计数试剂盒-8和集落形成试验检测GC细胞增殖能力。
PLOD3在人GC组织（与相邻正常组织相比，p < 0.001）和GC细胞中上调。PLOD3的高表达与更大的肿瘤大小（p = 0.007）和不良预后显著相关。抑制PLOD3可抑制GC中的细胞增殖。
这些结果表明PLOD3可能促进GC的进展。
