Choi Yong-Eui, Ahn Chang Ho, Kim Bo-Bae, Yoon Eui-Soo
Division of Forest Resources, Colleges of Forest Sciences, Kangwon National University, Chunchon 200-701, Korea.
Biol Pharm Bull. 2008 Jan;31(1):135-8. doi: 10.1248/bpb.31.135.
Panax japonicus is an important medicinal plant. The aim of this study was to develop species-specific molecular markers for P. japonicus. Amplified fragment length polymorphism (AFLP) was compared among P. japonicus, P. ginseng and P. quinquefolius. A clear species-specific AFLP marker for P. japonicus was generated. After isolation and sequencing of the AFLP fragment, a DNA sequence (293 bp) was obtained and named JG14. Oligonucleotide primer (23 mer) was designed for amplifying 191 bp of the sequence of JG14. PCR analysis revealed a clear amplified band for P. japonicus but not in 3 other Panax species (P. ginseng, P. quinquefolius and P. notoginseng). This sequence characterized amplified regions (SCAR) marker will be used for rapid authentication of P. japonicus among other related Panax species. This is the first report of species-specific SCAR marker development in P. japonicus.
竹节参是一种重要的药用植物。本研究的目的是开发竹节参的物种特异性分子标记。对竹节参、人参和西洋参进行了扩增片段长度多态性（AFLP）比较。获得了一个清晰的竹节参物种特异性AFLP标记。对AFLP片段进行分离和测序后，得到一个293 bp的DNA序列，命名为JG14。设计了寡核苷酸引物（23个碱基）用于扩增JG14序列的191 bp。PCR分析显示竹节参有一条清晰的扩增带，而在其他3种人参属植物（人参、西洋参和三七）中没有。这种序列特征性扩增区域（SCAR）标记将用于在其他相关人参属物种中快速鉴定竹节参。这是关于竹节参物种特异性SCAR标记开发的首次报道。
