Miswan N, Singham G V, Othman N
Centre for Chemical Biology, Universiti Sains Malaysia, 11900 Penang, Malaysia.
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Helminthologia. 2022 Dec 30;59(4):321-340. doi: 10.2478/helm-2022-0034. eCollection 2022 Dec.
World Health Organization (WHO) reported that over 1.5 billion people are infected by soil-transmitted helminths (STH) worldwide in sub-Saharan Africa, the United States of America, China, and East Asia. Heavy infections and polyparasitism are associated with higher morbidity rates, and the patients are exposed to increased vulnerability to other diseases. Therefore, accurate diagnosis followed by mass treatment for morbidity control is necessary.STH diagnosis commonly involves the microscopic observation of the presence of the STH eggs and larvae in the faecal samples. Furthermore, molecular approaches are increasingly utilised in monitoring and surveillance as they show higher sensitivity. Their capability to differentiate hookworm species is an advantage over the Kato-Katz technique. This review discusses the advantages and limitations of microscopy and various molecular tools used for STH detection.
世界卫生组织（WHO）报告称，在撒哈拉以南非洲、美利坚合众国、中国和东亚，全球有超过15亿人感染土源性蠕虫（STH）。重度感染和多重寄生虫感染与较高的发病率相关，患者更容易感染其他疾病。因此，为了控制发病率，进行准确诊断并随后开展大规模治疗是必要的。STH诊断通常包括对粪便样本中土源性蠕虫虫卵和幼虫的存在情况进行显微镜观察。此外，分子方法因其更高的灵敏度而越来越多地用于监测和 surveillance。它们区分钩虫种类的能力是优于加藤厚涂片法的一个优势。本综述讨论了用于STH检测的显微镜检查和各种分子工具的优缺点。
