Ribeiro Ana Cláudia Alves Freire, Batista Tatiane Helena, Veronesi Vanessa Barbosa, Giusti-Paiva Alexandre, Vilela Fabiana Cardoso
Departamento de Ciências Fisiológicas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (Unifal-MG), Alfenas, Minas Gerais, Brazil; Programa de Pós-Graduação em Biociências Aplicadas à Saúde, Brazil.
Departamento de Ciências Fisiológicas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (Unifal-MG), Alfenas, Minas Gerais, Brazil; Programa de Pós-Graduação em Biociências Aplicadas à Saúde, Brazil.
Int J Dev Neurosci. 2018 Aug;68:45-52. doi: 10.1016/j.ijdevneu.2018.05.001. Epub 2018 May 3.
The objective of this study was to investigate the effect of exposure to maternal consumption of a hyperenergetic, highly palatable diet, known as the cafeteria diet, during the gestation period on the development and behavior of offspring. For this, we used pregnant female mice that were fed a normal or a cafeteria diet during the gestation period. The evaluation of maternal behavior in lactating dams was performed from the second to the eighth day postpartum (PND 2-8). Weight gain, feed intake, and energy intake were recorded during the gestation period. In the offspring, reflex parameters and physical development were evaluated during the lactation period and when they reached adolescence. Behavioral performance was evaluated in light-dark, open-field, and play behavior tests. In addition, biochemical parameters of the dams and the adolescent offspring were evaluated. The cafeteria diet during gestation altered maternal behavior and the onset of physical and neurodevelopmental landmarks and had an impact on emotional and play behavior in adolescent offspring. In conclusion, our results demonstrate that exposure to maternal consumption of a cafeteria diet during the gestation period can program developmental and behavioral courses in the offspring.
本研究的目的是调查孕期母鼠食用高热量、高适口性饮食（即自助餐厅饮食）对后代发育和行为的影响。为此，我们使用了孕期分别喂食正常饮食或自助餐厅饮食的怀孕雌性小鼠。对哺乳期母鼠的母性行为评估在产后第二天至第八天（出生后第2 - 8天）进行。孕期记录体重增加、采食量和能量摄入量。在后代中，泌乳期以及达到青春期时评估反射参数和身体发育情况。通过明暗箱、旷场和玩耍行为测试评估行为表现。此外，还评估了母鼠和青春期后代的生化参数。孕期的自助餐厅饮食改变了母性行为以及身体和神经发育里程碑的出现时间，并对青春期后代的情绪和玩耍行为产生影响。总之，我们的结果表明，孕期母鼠食用自助餐厅饮食会影响后代的发育和行为进程。
