Zhao Zhi, Zhou Xinshe, Guan Jianzhong, Wu Min, Zhou Jiansheng
Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Anhui Key Laboratory of Tissue Transplantation, Bengbu, Anhui 233004, P.R. China.
Oncol Lett. 2018 May;15(5):7021-7027. doi: 10.3892/ol.2018.8195. Epub 2018 Mar 7.
Replacement of chondrocytes by adult stem cells was believed to improve the performance of autologous chondrocytes transplantation, since less chondrocytes were needed. Previous studies have demonstrated that the increased cartilage production in pellet co-cultures of chondrocytes and bone marrow mesenchymal stem cells (BMSCs) is due to the trophic effects of the MSC by stimulating chondrocyte proliferation and matrix production. However, the destination of MSCs or chondrocytes after implanted in osteo-chondral defects is not clear. The aim of the present study is to investigate the viability of MSCs and chondrocytes after co-implantation into a rat osteo-chondral defect model. MSCs were isolated from bone marrow and chondrocytes were extracted from knee joints of neonatal rats. Results of sulfated glycosaminoglycans (GAG) and collagen quantification demonstrated that co-culture pellets of BMSCs and chondrocytes have more GAG deposition than that of BMSCs or chondrocytes alone. Tracking cells with fluorescence protein demonstrated that MSCs disappeared following co-culture. In a rat knee injury model, co-implantation of BMSCs and chondrocytes contained more viable chondrocytes than chondrocytes implanted alone. To conclude, BMSCs were replaced by chondrocytes in pellet co-culture and BMSCs increased the viability of chondrocytes following co-implantation in a osteo-chondral defects model. Co-implantation of BMSCs and chondrocytes may be a promising approach to repairing osteo-chondral defects in the clinical setting.
由于所需软骨细胞较少，人们认为用成体干细胞替代软骨细胞可提高自体软骨细胞移植的效果。以往研究表明，软骨细胞与骨髓间充质干细胞（BMSC）的微团共培养中软骨生成增加是由于MSC通过刺激软骨细胞增殖和基质产生发挥了营养作用。然而，将MSC或软骨细胞植入骨软骨缺损后它们的去向尚不清楚。本研究的目的是探讨共植入大鼠骨软骨缺损模型后MSC和软骨细胞的存活情况。从骨髓中分离MSC，从新生大鼠膝关节中提取软骨细胞。硫酸化糖胺聚糖（GAG）和胶原蛋白定量结果表明，BMSC与软骨细胞的共培养微团比单独的BMSC或软骨细胞微团有更多的GAG沉积。用荧光蛋白追踪细胞表明，共培养后MSC消失。在大鼠膝关节损伤模型中，与单独植入软骨细胞相比，共植入BMSC和软骨细胞含有更多存活的软骨细胞。总之，在微团共培养中BMSC被软骨细胞替代，在骨软骨缺损模型中共植入后BMSC提高了软骨细胞的存活率。BMSC与软骨细胞的共植入可能是临床上修复骨软骨缺损的一种有前景的方法。
