Poroyko Valeriy, Mirzapoiazova Tamara, Nam Arin, Mambetsariev Isa, Mambetsariev Bolot, Wu Xiwei, Husain Aliya, Vokes Everett E, Wheeler Deric L, Salgia Ravi
Department of Medical Oncology and Therapeutics Research, City of Hope National Medical Center, Duarte, CA, USA.
Department of Molecular and Cellular Biology, City of Hope National Medical Center, Duarte, CA, USA.
Oncotarget. 2018 Apr 13;9(28):19793-19806. doi: 10.18632/oncotarget.24857.
Lung cancer is a devastating disease with overall bleak prognosis. Current methods to diagnose lung cancer are rather invasive and are inadequate to detect the disease at an early stage when treatment is likely to be most effective. In this study, a shotgun sequencing approach was used to study the microRNA (miRNA) cargo of serum-derived exosomes of small cell lung cancer (SCLC) (n=9) and non-small cell lung cancer (NSCLC) (n=11) patients, and healthy controls (n=10). The study has identified 17 miRNA species that are differentially expressed in cancer patients and control subjects. Furthermore, within the patient groups, a set of miRNAs were differentially expressed in exosomal samples obtained before and after chemotherapy treatment. This manuscript demonstrates the potential of exosomal miRNAs for developing noninvasive tests for disease differentiation and treatment monitoring in lung cancer patients.
肺癌是一种毁灭性疾病，总体预后不佳。目前诊断肺癌的方法具有相当的侵入性，并且不足以在治疗可能最有效的早期阶段检测出该疾病。在本研究中，采用鸟枪法测序方法研究了小细胞肺癌（SCLC）患者（n = 9）、非小细胞肺癌（NSCLC）患者（n = 11）和健康对照者（n = 10）血清来源外泌体的微小RNA（miRNA）成分。该研究鉴定出17种在癌症患者和对照受试者中差异表达的miRNA种类。此外，在患者组中，一组miRNA在化疗治疗前后获得的外泌体样本中差异表达。本论文证明了外泌体miRNA在开发用于肺癌患者疾病鉴别和治疗监测的非侵入性检测方法方面的潜力。
