Afridi Waqar Ahmed, Strachan Simon, Kasetsirikul Surasak, Pannu Amandeep Singh, Soda Narshone, Gough Daniel, Nguyen Nam-Trung, Shiddiky Muhammad J A
School of Environment and Science, Griffith University, Nathan Campus, Nathan, QLD 4111, Australia.
Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, Nathan, QLD 4111, Australia.
ACS Meas Sci Au. 2023 Feb 14;3(3):143-161. doi: 10.1021/acsmeasuresciau.2c00068. eCollection 2023 Jun 21.
Around the world, lung cancer has long been the main factor in cancer-related deaths, with small-cell lung cancer (SCLC) being the deadliest form of lung cancer. Cancer cell-derived exosomes and exosomal miRNAs are considered promising biomarkers for diagnosing and prognosis of various diseases, including SCLC. Due to the rapidity of SCLC metastasis, early detection and diagnosis can offer better diagnosis and prognosis and therefore increase the patient's chances of survival. Over the past several years, many methodologies have been developed for analyzing non-SCLC-derived exosomes. However, minimal advances have been made in SCLC-derived exosome analysis methodologies. This Review discusses the epidemiology and prominent biomarkers of SCLC. Followed by a discussion about the effective strategies for isolating and detecting SCLC-derived exosomes and exosomal miRNA, highlighting the critical challenges and limitations of current methodologies. Finally, an overview is provided detailing future perspectives for exosome-based SCLC research.
在全球范围内，肺癌长期以来一直是癌症相关死亡的主要因素，其中小细胞肺癌（SCLC）是最致命的肺癌形式。癌细胞衍生的外泌体和外泌体miRNA被认为是诊断和预测包括SCLC在内的各种疾病的有前景的生物标志物。由于SCLC转移迅速，早期检测和诊断可以提供更好的诊断和预后，从而增加患者的生存机会。在过去几年中，已经开发了许多用于分析非SCLC衍生外泌体的方法。然而，SCLC衍生外泌体分析方法的进展甚微。本综述讨论了SCLC的流行病学和突出的生物标志物。随后讨论了分离和检测SCLC衍生外泌体和外泌体miRNA的有效策略，强调了当前方法的关键挑战和局限性。最后，概述了基于外泌体的SCLC研究的未来前景。
