分别通过紫外线C光和亚甲蓝加可见光使血小板浓缩物和血浆中的埃博拉病毒和中东呼吸综合征冠状病毒失活。
Inactivation of Ebola virus and Middle East respiratory syndrome coronavirus in platelet concentrates and plasma by ultraviolet C light and methylene blue plus visible light, respectively.
作者信息
Eickmann Markus, Gravemann Ute, Handke Wiebke, Tolksdorf Frank, Reichenberg Stefan, Müller Thomas H, Seltsam Axel
机构信息
Institute for Virology, Philipps University Marburg, Marburg, Germany.
German Red Cross Blood Service NSTOB, Springe, Germany.
出版信息
Transfusion. 2018 Sep;58(9):2202-2207. doi: 10.1111/trf.14652. Epub 2018 May 6.
Abstract
BACKGROUND
Ebola virus (EBOV) and Middle East respiratory syndrome coronavirus (MERS-CoV) have been identified as potential threats to blood safety. This study investigated the efficacy of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate EBOV and MERS-CoV in platelet concentrates (PCs) and plasma, respectively.
STUDY DESIGN AND METHODS
PCs and plasma were spiked with high titers of cell culture-derived EBOV and MERS-CoV, treated with various light doses of ultraviolet C (UVC; THERAFLEX UV-Platelets) or methylene blue (MB) plus visible light (MB/light; THERAFLEX MB-Plasma), and assessed for residual viral infectivity.
RESULTS
UVC reduced EBOV (≥4.5 log) and MERS-CoV (≥3.7 log) infectivity in PCs to the limit of detection, and MB/light decreased EBOV (≥4.6 log) and MERS-CoV (≥3.3 log) titers in plasma to nondetectable levels.
CONCLUSIONS
Both THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce EBOV and MERS-CoV infectivity in platelets and plasma, respectively.
摘要
背景
埃博拉病毒（EBOV）和中东呼吸综合征冠状病毒（MERS-CoV）已被确定为对血液安全的潜在威胁。本研究分别调查了THERAFLEX UV-血小板和THERAFLEX MB-血浆病原体灭活系统对灭活血小板浓缩物（PCs）中的埃博拉病毒和血浆中的中东呼吸综合征冠状病毒的效果。
研究设计与方法
将高滴度的细胞培养来源的埃博拉病毒和中东呼吸综合征冠状病毒加入到PCs和血浆中，用不同光剂量的紫外线C（UVC；THERAFLEX UV-血小板）或亚甲蓝（MB）加可见光（MB/光；THERAFLEX MB-血浆）进行处理，并评估残余病毒感染性。
结果
UVC将PCs中的埃博拉病毒（≥4.5 log）和中东呼吸综合征冠状病毒（≥3.7 log）感染性降低至检测限，MB/光将血浆中的埃博拉病毒（≥4.6 log）和中东呼吸综合征冠状病毒（≥3.3 log）滴度降低至不可检测水平。
结论
THERAFLEX UV-血小板（UVC）和THERAFLEX MB-血浆（MB/光）分别有效降低血小板和血浆中的埃博拉病毒和中东呼吸综合征冠状病毒感染性。
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