Al-Neklawy Ahmed Farid
Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Clin Anat. 2018 Sep;31(6):830-837. doi: 10.1002/ca.23200. Epub 2018 Aug 22.
In this study, the protective effect of spirulina on corneal injury after formaldehyde (FA) exposure was assessed. Thirty adult male albino rats were divided into four groups. Group I: 12 rats were divided into two subgroups: I-a (negative control) and I-b (positive control). Group II (spirulina group): six rats received spirulina via an oral gavage feeding needle at a daily dose of 400 mg/kg b.w. Group III (FA exposure group): six rats were subjected to 10% FA inhalation for 2 h per day for 2 weeks (5 days per week). Group IV (FA exposure group treated with spirulina): six rats were exposed to 10% FA as in group III, with co-administration of spirulina as in group II. After 2 weeks, all the rats were sacrificed; the corneas were dissected and processed for paraffin sections. The sections were stained with hematoxylin and eosin (H&E), Masson's trichrome (MTC), or avidin-biotin peroxidase, and examined by light microscopy. The sections of rat cornea exposed to FA (Group III) showed disorganized and compressed epithelium with erosions. Subepithelial mononuclear cell infiltration and invasion of blood vessels were also evident. Stromal collagen fibers were disorganized and widely separated. All these changes were ameliorated by administration of spirulina (Group IV). Corneal thickness was nearly normal in Group IV, statistically significantly less than in Group III. It was concluded that spirulina protects against FA-induced corneal injury in rats. Clin. Anat. 31:830-837, 2018. © 2018 Wiley Periodicals, Inc.
在本研究中，评估了螺旋藻对甲醛（FA）暴露后角膜损伤的保护作用。30只成年雄性白化大鼠被分为四组。第一组：12只大鼠分为两个亚组：I-a（阴性对照）和I-b（阳性对照）。第二组（螺旋藻组）：6只大鼠通过口服灌胃针接受螺旋藻，每日剂量为400mg/kg体重。第三组（FA暴露组）：6只大鼠每天接受10%FA吸入2小时，持续2周（每周5天）。第四组（用螺旋藻治疗的FA暴露组）：6只大鼠如第三组一样暴露于10%FA，同时如第二组一样给予螺旋藻。2周后，处死所有大鼠；解剖角膜并制作石蜡切片。切片用苏木精和伊红（H&E）、Masson三色染色法（MTC）或抗生物素蛋白-生物素过氧化物酶染色，并通过光学显微镜检查。暴露于FA的大鼠角膜切片（第三组）显示上皮组织紊乱、压缩且有糜烂。上皮下单核细胞浸润和血管侵入也很明显。基质胶原纤维紊乱且广泛分离。给予螺旋藻（第四组）后，所有这些变化均得到改善。第四组角膜厚度接近正常，统计学上显著低于第三组。得出的结论是，螺旋藻可保护大鼠免受FA诱导的角膜损伤。《临床解剖学》31:830 - 837，2018年。©2018威利期刊公司。
