Ballesteros D, Pence V C
Center for Research of Endangered Wildlife (CREW). Cincinnati Zoo and Botanical Garden， Cincinnati, OH, USA. Department of Comparative Plant and Fungal Biology, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex, United Kingdom.
Center for Research of Endangered Wildlife (CREW). Cincinnati Zoo and Botanical Garden， Cincinnati, OH, USA.
Cryo Letters. 2017 Jul/Aug;38(4):278-289.
The low temperature of liquid nitrogen is assumed to stop ageing and preserve viability indefinitely, however there are few validating data sets. The use of seeds to test these assumptions is important because other cryopreserved systems lack quantitative measures of viability to allow comparisons among timed points.
To evaluate survival of a collection of seeds with short lifespans stored 12-20 years in liquid nitrogen.
Seeds from 11 species (26 accessions) were removed from cryostorage and evaluated for germination and normal growth.
Germination of Plantago cordata and Betula spp. seeds did not decrease significantly during cryostorage. However, Populus deltoides and most Salix spp. accessions showed a significant decrease in germination, with further loss observed when P. deltoides seedlings were followed to the young plant stage. Seeds of initial low quality showed greater deterioration during cryostorage.
Cryostorage maintained viability of Salix and Populus seeds longer than other temperatures. However, ageing was not completely stopped and seed longevity was shorter than that predicted for many other species. A high initial seed quality is important in order to obtain the maximum benefit of cryostorage.
液氮的低温被认为可以停止老化并无限期地保持活力，然而，验证数据集很少。使用种子来检验这些假设很重要，因为其他冷冻保存系统缺乏活力的定量测量方法，无法在不同时间点之间进行比较。
评估在液氮中储存12 - 20年的一批短寿命种子的存活率。
从低温储存中取出11个物种（26个种质）的种子，评估其发芽和正常生长情况。
心叶车前和桦木属种子在低温储存期间发芽率没有显著下降。然而，三角叶杨和大多数柳树种质的发芽率显著下降，当三角叶杨幼苗进入幼龄植株阶段时，进一步观察到发芽率损失。初始质量较低的种子在低温储存期间劣化程度更大。
低温储存比其他温度更能长时间保持柳树和杨树种子的活力。然而，老化并没有完全停止，种子寿命比许多其他物种预测的要短。为了从低温储存中获得最大益处，种子初始质量高很重要。
