Dalziell Emma L, Funnekotter Bryn, Mancera Ricardo L, Merritt David J
Kings Park Science, Department of Biodiversity, Conservation and Attractions, Kings Park, WA, Australia.
School of Biological Sciences, The University of Western Australia, Crawley, WA, Australia.
Conserv Physiol. 2019 May 10;7(1):coz021. doi: 10.1093/conphys/coz021. eCollection 2019.
Eighteen native species of (waterlilies) inhabit a range of freshwater wetlands in northern Australia, which are threatened by increased development and the potential impacts of climate change. To investigate conservation seed banking of these vulnerable species, we aimed to characterize their seed storage physiology by determining (i) seed desiccation tolerance and (ii) the effects of moisture content and storage temperature on seed germination and viability. Seeds of , , and (including multiple collections of three species) were placed in experimental storage at a range of temperatures (25°C, 5°C, -20°C and -190°C) following pre-equilibration at different RHs (15%, 30%, 50%, 70% or 95%). Seeds were also experimentally aged at 60% RH and 45°C to assess comparative longevity. We found seeds of all species to be desiccation tolerant. However, the responses of seeds to experimental storage conditions were complex and variable between species and collections of the same species, and seeds of many species/collections were short-lived across many of the storage treatments. In many cases decreasing storage temperature did not increase longevity. Additional protocol development is necessary before we can have confidence that seed banking is a viable long-term germplasm conservation strategy for .
18种睡莲原生种栖息在澳大利亚北部一系列淡水湿地中，这些湿地受到开发增加和气候变化潜在影响的威胁。为了研究这些濒危物种的种子库保存，我们旨在通过确定（i）种子脱水耐受性以及（ii）水分含量和储存温度对种子萌发和活力的影响来表征其种子储存生理特性。在不同相对湿度（15%、30%、50%、70%或95%）下预平衡后，将睡莲属、芡实属、王莲属和萍蓬草属的种子（包括三个物种的多个采集样本）置于一系列温度（25°C、5°C、-20°C和-190°C）的实验储存条件下。种子还在60%相对湿度和45°C条件下进行人工老化处理，以评估相对寿命。我们发现所有物种的种子都具有脱水耐受性。然而，种子对实验储存条件的反应很复杂，物种之间以及同一物种的不同采集样本之间存在差异，许多物种/采集样本的种子在许多储存处理中寿命较短。在许多情况下，降低储存温度并不会延长种子寿命。在我们能够确信种子库保存是睡莲可行的长期种质保存策略之前，还需要进一步制定方案。
