Acosta Y, Hernandez L, Mazorra C, Quintana N, Zevallos B E, Cejas I, Sershen EmptyYN Y, Lorenzo J C, Martínez-Montero M E, Fontes D
Faculty of Agricultural Sciences, University of Ciego de Ávila, Ciego de Ávila, Cuba.
Laboratory for Plant Breeding & Conservation of Genetic Resources, Bioplant Center, University of Ciego de Ávila, Ciego de Ávila, Cuba.
Cryo Letters. 2019 Jan/Feb;40(1):36-44.
Teramnus labialis is an herbaceous legume that serves as a source of carbohydrates and proteins for animals and humans, and is valued for its nitrogen contribution to soil. The benefits of this species are, however, limited by low seed availability, small seed size and low in situ seed germination levels, due to physical dormancy. Cryostorage has been shown to be beneficial for both seed storage and breaking physical dormancy in seeds of various species. However, its potential effects on subsequent seedling emergence, plant growth and seed production need to be studied before large-scale implementation for T. labialis.
To record agricultural traits of T. labialis after seed exposure to liquid nitrogen.
Seeds were maintained at 5 degree C (control) or stored in LN before sowing. Seedling emergence percentage and traits related to plant growth and seed production were evaluated for 6 months.
Except for seed weight, all traits differed significantly between seedlings generated from cryostored and control seeds. Except for pod number, seedling emergence and plant growth traits were enhanced by cryostorage to a greater extent than seed production traits. Cryostorage resulted in cracks and breaks in the seed coat which were absent in control seeds (scanning electron microscopy), and in breaking physical dormancy may have facilitated more rapid seedling emergence than for control seeds.
Seed cryostorage enhances subsequent plant productivity in terms of growth and to a lesser extent seed production in Teramnus labialis, validating its use for commercial growth of this species.
唇萼田菁是一种草本豆科植物，可为动物和人类提供碳水化合物和蛋白质来源，并且因其对土壤的氮贡献而受到重视。然而，由于物理休眠，该物种的益处受到种子可用性低、种子体积小和原地种子发芽水平低的限制。低温贮藏已被证明对各种物种的种子贮藏和打破种子的物理休眠都有益。然而，在大规模应用于唇萼田菁之前，需要研究其对后续幼苗出土、植物生长和种子生产的潜在影响。
记录唇萼田菁种子经液氮处理后的农艺性状。
种子在5℃下保存（对照）或在播种前贮藏于液氮中（液氮处理）。对幼苗出土率以及与植物生长和种子生产相关的性状进行了6个月的评估。
除种子重量外，经低温贮藏的种子和对照种子产生的幼苗在所有性状上均存在显著差异。除荚果数外，低温贮藏对幼苗出土和植物生长性状的促进作用大于对种子生产性状的促进作用。低温贮藏导致种皮出现裂缝和破损，而对照种子没有（扫描电子显微镜观察），并且打破物理休眠可能使幼苗出土比对照种子更快。
种子低温贮藏提高了唇萼田菁后续的植物生长生产力，在一定程度上也提高了种子产量，证实了其在该物种商业种植中的应用价值。
