Palmerini Maria Grazia, Antonouli Sevastiani, Macchiarelli Guido, Cecconi Sandra, Bianchi Serena, Khalili Mohammad Ali, Nottola Stefania Annarita
Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Department of Reproductive Biology, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd 97514, Iran.
Cells. 2022 May 13;11(10):1636. doi: 10.3390/cells11101636.
After its discovery in 1825 by the physiologist J.E. Purkinje, the human germinal vesicle (GV) attracted the interest of scientists. Discarded after laparotomy or laparoscopic ovum pick up from the pool of retrieved mature oocytes, the leftover GV was mainly used for research purposes. After the discovery of Assisted Reproductive Technologies (ARTs) such as maturation (IVM), fertilization and embryo transfer (IVF-ET) and intracytoplasmic sperm injection (ICSI), its developing potential was explored, and recognized as an important source of germ cells, especially in the case of scarce availability of mature oocytes for pathological/clinical conditions or in the case of previous recurrent implantation failure. We here review the ultrastructural data available on GV-stage human oocytes and their application to ARTs.
1825年生理学家J.E. 浦肯野发现人类生发泡（GV）后，它便引起了科学家们的兴趣。在剖腹手术或从回收的成熟卵母细胞池中进行腹腔镜取卵后被丢弃的剩余GV，主要用于研究目的。在发现诸如成熟（IVM）、受精和胚胎移植（IVF-ET）以及卵胞浆内单精子注射（ICSI）等辅助生殖技术（ARTs）后，人们对其发育潜力进行了探索，并将其视为生殖细胞的重要来源，尤其是在病理/临床情况下成熟卵母细胞供应稀缺或先前反复植入失败的情况下。我们在此回顾关于GV期人类卵母细胞的现有超微结构数据及其在ARTs中的应用。
