视神经炎与基因单核苷酸多态性及白细胞介素-17A浓度的关联
Association of Optic Neuritis with Gene Single Nucleotide Polymorphism and IL-17A Concentration.
作者信息
Banevicius Mantas, Vilkeviciute Alvita, Glebauskiene Brigita, Kriauciuniene Loresa, Liutkeviciene Rasa
机构信息
Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, Eiveniu 2, LT-50161 Kaunas, Lithuania.
Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Eiveniu 2, LT-50161 Kaunas, Lithuania.
出版信息
J Ophthalmol. 2018 Mar 15;2018:1686297. doi: 10.1155/2018/1686297. eCollection 2018.
BACKGROUND
The aetiology and pathophysiology of optic neuritis (ON) is not absolutely clear but genetic and inflammatory factors may be also involved in its development. The aim of the present study was to determine the influence of single nucleotide polymorphism (SNP) of (rs1558139) and serum levels of IL-17A on ON development.
MATERIALS AND METHODS
Forty patients with ON and 164 control subjects were evaluated. Patients were divided by gender, also ON patients were divided into two subgroups: ON with and without multiple sclerosis (MS). rs1558139 was genotyped using real-time PCR. Serum IL-17A levels were measured using ELISA IL-17A kits.
RESULTS
We found that A/A genotype of rs1558139 was statistically significantly more frequent in men with ON and MS than in women: 57.1% versus 0%, = 0.009. Also, allele A was statistically significantly more frequent in men with ON and MS than in women: 71.4% versus 37.5%, = 0.044. Serum levels of IL-17A were higher in ON group than in control group: (median, IQR): 20.55 pg/ml, 30.66 pg/ml versus 8.97 pg/ml, 6.24 pg/ml, < 0.001.
CONCLUSION
The higher IL-17A levels were found to be associated with ON, while allele A at rs1558139 was associated only with ON with MS in male patients.
背景
视神经炎（ON）的病因和病理生理学尚未完全明确，但遗传和炎症因素可能也参与其发病过程。本研究的目的是确定（rs1558139）单核苷酸多态性（SNP）和血清白细胞介素-17A（IL-17A）水平对视神经炎发病的影响。
材料与方法
对40例视神经炎患者和164例对照者进行评估。患者按性别分组，视神经炎患者也分为两个亚组：伴和不伴多发性硬化（MS）的视神经炎患者。采用实时聚合酶链反应（PCR）对rs1558139进行基因分型。使用ELISA IL-17A试剂盒检测血清IL-17A水平。
结果
我们发现，rs1558139的A/A基因型在患有视神经炎和多发性硬化的男性中比女性更常见，差异有统计学意义：57.1% 对0%，P = 0.009。此外，等位基因A在患有视神经炎和多发性硬化的男性中比女性更常见，差异有统计学意义：71.4% 对37.5%，P = 0.044。视神经炎组血清IL-17A水平高于对照组：（中位数，四分位间距）：20.55 pg/ml，30.66 pg/ml对8.97 pg/ml，6.24 pg/ml，P < 0.001。
结论
发现较高的IL-17A水平与视神经炎有关，而rs1558139位点的A等位基因仅与男性患者中伴有多发性硬化的视神经炎有关。
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