Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Division of Periodontology, College of Dentistry, The Ohio State University, Columbus, Ohio.
J Clin Periodontol. 2018 Aug;45(8):932-940. doi: 10.1111/jcpe.12908. Epub 2018 Jun 25.
Periodontitis is correlated with type 2 diabetes mellitus (T2DM), but little is known about glycaemic status effect on subgingival microbiota associated with periodontitis. This study evaluated if periodontal microbiome of T2DM patients is affected by glycaemic status.
Twenty-one T2DM non-smoking patients with chronic periodontitis and body mass index ≤40 kg/m were allocated into two groups according to systemic glycaemic status: inadequate (DMI- HbA1c ≥ 8%) and adequate (DMA- HbA1c <7.8%). Subgingival biofilm was collected from sites with moderate (PD = 4-6 mm) and severe disease (PD ≥ 7 mm) in two quadrants. The V5-V6 hypervariable region of the 16SrRNA was sequenced using the GS-FLX-454 Titanium platform. Sequences were compared with HOMD database using QIIME and PhyloToAST pipelines. Statistical comparisons were made using two-sample t-tests.
DMA microbiome presented higher diversity than DMI. Inadequate glycaemic control favoured fermenting species, especially those associated with propionate/succinate production, whereas those forming butyrate/pyruvate was decreased in DMI. Higher abundances of anginosus group and Streptococcus agalactiae in DMI may indicate that subgingival sites can be reservoir of potentially invasive pathogens. Altered subgingival microbiome in DMI may represent an additional challenge in the periodontal treatment of these patients and in the prevention of more invasive infections.
Glycaemic status in T2DM patients seems to modulate subgingival biofilm composition.
牙周炎与 2 型糖尿病（T2DM）相关，但对于血糖状态对与牙周炎相关的龈下微生物群的影响知之甚少。本研究评估了 T2DM 患者的牙周微生物组是否受到血糖状态的影响。
将 21 名患有慢性牙周炎且体重指数（BMI）≤40kg/m²的非吸烟 T2DM 患者根据全身血糖状态分为两组：血糖控制不充分（DMI-HbA1c≥8%）和血糖控制充分（DMA-HbA1c<7.8%）。从两个象限中具有中度（PD=4-6mm）和重度疾病（PD≥7mm）的部位采集龈下生物膜。使用 GS-FLX-454 Titanium 平台对 16SrRNA 的 V5-V6 高变区进行测序。使用 QIIME 和 PhyloToAST 管道将序列与 HOMD 数据库进行比较。使用两样本 t 检验进行统计比较。
DMA 微生物组的多样性高于 DMI。血糖控制不充分有利于发酵物种，特别是那些与丙酸/琥珀酸产生相关的物种，而在 DMI 中，形成丁酸/丙酮酸的物种减少。DMI 中龈下部位可能是潜在侵袭性病原体的储库，放线菌属和无乳链球菌的丰度较高可能表明。DMI 中改变的龈下微生物组可能代表这些患者牙周治疗和预防更具侵袭性感染的额外挑战。
T2DM 患者的血糖状态似乎调节龈下生物膜组成。
