Zhou Chunhua, Jian Shaoqing, Peng Weidong, Li Min
School of Life Science, Nanchang University, Nanchang 330031, People's Republic of China.
College of Basic Medicine, Nanchang University, Nanchang 330031, People's Republic of China.
Korean J Parasitol. 2018 Apr;56(2):175-181. doi: 10.3347/kjp.2018.56.2.175. Epub 2018 Apr 30.
The giant roundworm infects pigs and people worldwide and causes serious diseases. The taxonomic relationship between and is still unclear. The purpose of the present study was to investigate the genetic diversity and population genetic structure of 258 specimens from humans and pigs from 6 sympatric regions in -endemic regions of China using existing simple sequence repeat data. The microsatellite markers showed a high level of allelic richness and genetic diversity in the samples. Each of the populations demonstrated excess homozygosity (Ho<He, Fis>0). According to a genetic differentiation index (Fst=0.0593), there was a high-level of gene flow in the populations. A hierarchical analysis on molecular variance revealed remarkably high levels of variation within the populations. Moreover, a population structure analysis indicated that populations fell into 3 main genetic clusters, interpreted as , , and a hybrid of the species. We speculated that humans can be infected with , , and the hybrid, but pigs were mainly infected with . This study provided new information on the genetic diversity and population structure of from human and pigs in China, which can be used for designing control strategies. It can also be beneficial to understand the introgression of host affiliation.
巨型蛔虫在全球范围内感染猪和人类，并引发严重疾病。[文中未提及的两个物种]与巨型蛔虫之间的分类关系仍不明确。本研究的目的是利用现有的简单序列重复数据，调查来自中国[地区名称未给出]流行地区6个同域地区的258份人类和猪的巨型蛔虫样本的遗传多样性和群体遗传结构。微卫星标记在样本中显示出高水平的等位基因丰富度和遗传多样性。每个群体都表现出纯合子过剩（Ho<He，Fis>0）。根据遗传分化指数（Fst = 0.0593），巨型蛔虫群体中存在高水平的基因流。分子方差的层次分析显示群体内部存在非常高的变异水平。此外，群体结构分析表明，巨型蛔虫群体分为3个主要的遗传簇，分别解释为[文中未提及的三个物种或类型]以及该物种的杂交种。我们推测人类可能感染[文中未提及的三个物种或类型]及其杂交种，但猪主要感染[文中未提及的某个物种或类型]。本研究提供了关于中国人类和猪源巨型蛔虫遗传多样性和群体结构的新信息，可用于设计巨型蛔虫控制策略。这对于理解宿主归属的基因渗入也可能有益。
