Shamshina Julia L, Zavgorodnya Oleksandra, Rogers Robin D
Mari Signum, Mid-Atlantic, Rockville, MD, USA.
525 Solutions, Inc., Tuscaloosa, AL, USA.
Adv Biochem Eng Biotechnol. 2019;168:177-198. doi: 10.1007/10_2018_63.
Chitin isolated through microwave-assisted dissolution using ionic liquids is a high molecular weight (MW) polymer that can be manufactured into materials of different architectures (e.g., fibers, films, microspheres, nanostructured materials) to be used as wound care dressings, drug delivery devices, scaffolds, etc. However, because of differences from traditional isolation methods and, thus, differences in polymer length and degree of deacetylation, it could exhibit bio-related properties that differ from those of traditionally 'pulped' chitin. Here we present the initial assessments of bio-related chitin properties in order to provide a useful scientific basis for clinical applications: biocompatibility, cytotoxicity (intracutaneous reactivity), wound healing efficacy, histological evaluation of the wounds treated with chitin dressing, and antibacterial activity. We also provide the studies that outline potential applications of chitin as a raw polymer for preparation of biomaterials. Graphical Abstract.
通过使用离子液体的微波辅助溶解分离出的几丁质是一种高分子量聚合物，可制成不同结构的材料（如纤维、薄膜、微球、纳米结构材料），用作伤口护理敷料、药物递送装置、支架等。然而，由于与传统分离方法不同，因此聚合物长度和脱乙酰度存在差异，它可能表现出与传统“制浆”几丁质不同的生物相关特性。在此，我们展示了对生物相关几丁质特性的初步评估，以便为临床应用提供有用的科学依据：生物相容性、细胞毒性（皮内反应性）、伤口愈合效果、用几丁质敷料治疗的伤口的组织学评估以及抗菌活性。我们还提供了概述几丁质作为制备生物材料的原料聚合物的潜在应用的研究。图形摘要。
