Lummus Technology, Houston, TX 77086, USA.
InBio, Charlottesville, VA 22903, USA.
Molecules. 2022 Jun 21;27(13):3983. doi: 10.3390/molecules27133983.
The isolation of chitin utilizing ionic liquid 1-ethyl-3-methylimidazolium acetate has been determined to result in polymer contaminated with proteins. For the first time, the proteins in chitin extracted with ionic liquid have been quantified; the protein content was found to vary from 1.3 to 1.9% of the total weight. These proteins were identified and include allergenic proteins such as tropomyosin. In order to avoid 'traditional' hydroxide-based deproteinization of chitin, which could reduce the molecular weight of the final product, alternative deproteinization strategies were attempted. Testing of the previously reported deproteinization method using aqueous KPO resulted in protein reduction by factors varying from 2 to 10, but resulted in significant phosphate salt contamination of the final product. Contrarily, the incorporation of GRAS (Generally Recognized as Safe) compound Polysorbate 80 into the polymer washing step provided the polymer of comparable purity with no contaminants. This study presents new options for the deproteinization of chitin that can replace traditional approaches with methods that are environmentally friendly and can produce high purity polymer.
利用离子液体 1-乙基-3-甲基咪唑醋酸盐分离壳聚糖已被确定会导致聚合物被蛋白质污染。本文首次对离子液体提取的壳聚糖中的蛋白质进行了定量分析；发现蛋白质含量占总重量的 1.3%至 1.9%。这些蛋白质已被鉴定，其中包括过敏原蛋白，如原肌球蛋白。为了避免“传统”的基于氢氧化物的壳聚糖脱蛋白方法，因为该方法可能会降低最终产物的分子量，所以尝试了替代的脱蛋白策略。使用水性 KPO 对先前报道的脱蛋白方法进行测试，结果导致蛋白质的减少因子从 2 到 10 不等，但最终产物中磷酸盐盐的污染非常严重。相反，将 GRAS（一般认为安全）化合物聚山梨酯 80 掺入聚合物洗涤步骤中，提供了具有相同纯度且无污染物的聚合物。本研究为壳聚糖的脱蛋白提供了新的选择方案，可以用环保的方法替代传统方法，从而生产出高纯度的聚合物。
