Saha Tilak, Chakraborty Biswanath, Das Sayak, Thakur Nagendra, Chakraborty Ranadhir
Department of Biotechnology, OMICS Laboratory, University of North Bengal, Siliguri 734 013, West Bengal, India.
Department of Zoology, Microbiology and Immunology Laboratory, University of North Bengal, Siliguri 734 013, West Bengal, India.
Int J Syst Evol Microbiol. 2018 Jul;68(7):2165-2171. doi: 10.1099/ijsem.0.002791. Epub 2018 May 11.
A Gram-stain-positive, rod-shaped, slightly halotolerant, nitrate-reducing bacterial strain, designated ET03, was isolated from the cast of an earthworm (Eisenia fetida) reared at the Centre of Floriculture and Agribusiness Management, University of North Bengal at Siliguri, West Bengal, India. On the basis of 16S rRNA gene sequence phylogeny, the closest relative of strain ET03 was Chryseomicrobium palamuruense PU1 (99.1 % similarity). The DNA G+C content of strain ET03 was 42.9 mol%. Strain ET03 contained menaquinone-8 as the most predominant menaquinone and phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and phosphatidylglycerol as the main polar lipids. The diagnostic diamino acid was meso-diaminopimelic acid. Major cellular fatty acids were iso-C15 : 0, C16 : 1ω7c alcohol and iso-C16 : 0. Other biochemical and physiological analyses supported genotypic and phenotypic differentiation of the strain ET03 from its nearest taxonomic neighbours: Chryseomicrobium palamuruense,Chryseomicrobium amylolyticum, Chryseomicrobium imtechense, Chryseomicrobium aureum and Chryseomicrobium deserti. The draft genome of strain ET03 consisted of 2.64 Mb distributed in 14 scaffolds (N50 894072). A total of 2728 genes were predicted and, of those, 2664 were protein-coding genes including genes involved in the degradation of polychlorinated biphenyl and several aromatic compounds. The isolate, therefore, represents a novel species, for which the name Chryseomicrobium excrementi sp. nov. is proposed. The type strain is ET03 (=KCTC 33943=LMG 30119=JCM 32415).
从印度西孟加拉邦西里古里北孟加拉大学花卉与农业综合企业管理中心饲养的蚯蚓（赤子爱胜蚓）粪便中分离出一株革兰氏阳性、杆状、稍耐盐、能还原硝酸盐的细菌菌株，命名为ET03。基于其16S rRNA基因序列系统发育分析，菌株ET03的最相近亲缘种是帕拉穆鲁金色微杆菌PU1（相似度为99.1%）。菌株ET03的DNA G+C含量为42.9 mol%。菌株ET03以甲基萘醌-8作为最主要的甲基萘醌，以磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和磷脂酰甘油作为主要极性脂质。诊断性二氨基酸为内消旋二氨基庚二酸。主要细胞脂肪酸为异C15:0、C16:1ω7c醇和异C16:0。其他生化和生理分析支持了菌株ET03与其最近的分类学近缘种帕拉穆鲁金色微杆菌、解淀粉金色微杆菌、伊姆特琴金色微杆菌、金色金色微杆菌和沙漠金色微杆菌在基因型和表型上的差异。菌株ET03的基因组草图由2.64 Mb组成，分布在14个支架中（N50为894072）。共预测到2728个基因，其中2664个为蛋白质编码基因，包括参与多氯联苯和几种芳香化合物降解的基因。因此，该分离株代表一个新物种，提议将其命名为粪便金色微杆菌（Chryseomicrobium excrementi sp. nov.）。模式菌株为ET03（=KCTC 33943=LMG 30119=JCM 32415）。
