Saha Tilak, Ranjan Vivek K, Ganguli Sriradha, Thakur Subarna, Chakraborty Biswanath, Barman Partha, Ghosh Wriddhiman, Chakraborty Ranadhir
1OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri 734 013, West Bengal, India.
2Laboratory of Immunology, Department of Zoology, University of North Bengal, Siliguri 734 013, West Bengal, India.
Int J Syst Evol Microbiol. 2019 May;69(5):1265-1273. doi: 10.1099/ijsem.0.003304. Epub 2019 Feb 25.
A Gram-stain-positive, spore-forming bacterium, EAG3, capable of growing on 3-nitropropionic acid as the sole source of carbon, nitrogen and energy, was isolated from the anterior gut of an earthworm (Eisenia fetida) reared at the Centre of Floriculture and Agribusiness Management of the University of North Bengal at Siliguri (26.7072° N, 88.3558° E), West Bengal, India. The DNA G+C content of strain EAG3 was 42.5 mol%. Strain EAG3 contained MK-7 and MK-8 as predominant menaquinones. The predominant polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were 13-methyltetradecanoic acid, (9Z)-9-hexadecen-1-ol, 12-methyltetradecanoic acid and 14-methylpentadecanoic acid. The draft genome of strain EAG3, distributed in 57 contigs, was found to be 3.8 Mb. A total of 3811 potential coding sequences or genes were predicted, including 3672 protein-coding and 108 RNA-coding ones together with 31 pseudogenes. One hundred and thirty-five genes encoded hypothetical proteins with no meaningful homologies with known proteins. The EAG3 genome encompassed two nitronate monooxygenase and one methylmalonate-semialdehyde dehydrogenase (CoA acylating) homologues. 16S rRNA gene sequence-based phylogeny revealed that the closest relative of strain EAG3 was Bacillus methanolicus NCIMB 13113 (95.7 % similarity). Phylogenetic, physiological and biochemical characteristics differentiated strain EAG3 from B. methanolicus, as well as from the other close taxonomic relatives Planococcus rifietoensis M8, Bhargavaea cecembensis DSE10, Planomicrobium flavidum ISL-41and Fermentibacilluspolygoni IEB3, with which strain EAG3 had 93.3-94.2 % 16S rRNA gene sequence similarities. The new isolate, therefore, was considered as representing a novel genus of family Bacillaceae, for which the name Pradoshia eiseniae gen. nov., sp. nov. is proposed, with EAG3 (=LMG 30312=JCM 32460) as the type strain.
从印度西孟加拉邦西里古里（北纬26.7072°，东经88.3558°）北孟加拉大学花卉与农业综合企业管理中心饲养的蚯蚓（赤子爱胜蚓）前肠中分离出一株革兰氏阳性、形成芽孢的细菌EAG3，它能够以3-硝基丙酸作为唯一碳源、氮源和能源生长。菌株EAG3的DNA G+C含量为42.5 mol%。菌株EAG3以MK-7和MK-8作为主要甲基萘醌。主要极性脂为磷脂酰甘油、二磷脂酰甘油和磷脂酰乙醇胺。主要细胞脂肪酸为13-甲基十四烷酸、(9Z)-9-十六碳烯-1-醇、12-甲基十四烷酸和14-甲基十五烷酸。菌株EAG3的基因组草图分布在57个重叠群中，大小为3.8 Mb。共预测到3811个潜在编码序列或基因，包括3672个蛋白质编码基因、108个RNA编码基因以及31个假基因。135个基因编码与已知蛋白质无明显同源性的假定蛋白。EAG3基因组包含两个亚硝酸单加氧酶和一个丙二酸半醛脱氢酶（辅酶A酰化）同源物。基于16S rRNA基因序列的系统发育分析表明，菌株EAG3的最亲近亲缘种是甲醇芽孢杆菌NCIMB 13113（相似性为95.7%）。系统发育、生理和生化特征将菌株EAG3与甲醇芽孢杆菌以及其他近缘分类亲缘种，如里菲托球菌M8、切森巴格瓦菌DSE10、淡黄扁平微球菌ISL-41和多角发酵杆菌IEB3区分开来，菌株EAG3与这些菌株的16S rRNA基因序列相似性为93.3 - 94.2%。因此，该新分离株被认为代表芽孢杆菌科的一个新属，为此提出新属名Pradoshia eiseniae gen. nov.，新种名Pradoshia eiseniae sp. nov.，以EAG3（=LMG 30312=JCM 32460）作为模式菌株。
