转录组学和代谢组学分析表明，在大豆孢囊线虫感染大豆期间，细菌促进了植物的防御。
Transcriptomic and metabolomic analyses reveal that bacteria promote plant defense during infection of soybean cyst nematode in soybean.
机构信息
Nematology Institute of Northern China, Shenyang Agricultural University, No.120 Dongling Road, Shenyang, 110866, China.
Institute of Biotechnology, Shenyang Agricultural University, No.120 Dongling Road, Shenyang, 110866, China.
出版信息
BMC Plant Biol. 2018 May 11;18(1):86. doi: 10.1186/s12870-018-1302-9.
BACKGROUND
Soybean cyst nematode (SCN) is the most devastating pathogen of soybean. Our previous study showed that the plant growth-promoting rhizobacterium Bacillus simplex strain Sneb545 promotes soybean resistance to SCN. Here, we conducted a combined metabolomic and transcriptomic analysis to gain information regarding the biological mechanism of defence enhancement against SCN in Sneb545-treated soybean. To this end, we compared the transcriptome and metabolome of Sneb545-treated and non-treated soybeans under SCN infection.
RESULTS
Transcriptomic analysis showed that 6792 gene transcripts were common in Sneb545-treated and non-treated soybeans. However, Sneb545-treated soybeans showed a higher concentration of various nematicidal metabolites, including 4-vinylphenol, methionine, piperine, and palmitic acid, than non-treated soybeans under SCN infection.
CONCLUSIONS
Overall, our results validated and expanded the existing models regarding the co-regulation of gene expression and metabolites in plants, indicating the advantage of integrated system-oriented analysis.
背景
大豆胞囊线虫（SCN）是大豆最具破坏性的病原体。我们之前的研究表明，植物促生根瘤菌枯草芽孢杆菌 Sneb545 可促进大豆对 SCN 的抗性。在这里，我们进行了联合代谢组学和转录组学分析，以获得关于 Sneb545 处理的大豆增强对 SCN 防御的生物学机制的信息。为此，我们比较了 SCN 感染下 Sneb545 处理和未处理大豆的转录组和代谢组。
结果
转录组分析表明，Sneb545 处理和未处理大豆中有 6792 个基因转录本是共同的。然而，在 SCN 感染下，Sneb545 处理的大豆比未处理的大豆表现出更高浓度的各种杀线虫代谢物，包括 4-乙烯基苯酚、蛋氨酸、胡椒碱和棕榈酸。
结论
总的来说，我们的结果验证并扩展了现有关于植物中基因表达和代谢物的共同调控的模型，表明了综合系统导向分析的优势。
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