Hu Yi, Ma Yibing, Wang Liyi, Luo Qingqing, Zhao Zengqi, Wang Jianming, Xu Yumei
Laboratory of Nematology, Department of Plant Pathology, College of Plant Protection, Shanxi Agricultural University, Jinzhong, China.
Inveterate Group, Systematics, Manaaki Whenua-Landcare Research, Auckland, New Zealand.
Front Plant Sci. 2024 Sep 23;15:1458330. doi: 10.3389/fpls.2024.1458330. eCollection 2024.
Soybean cyst nematode (SCN) poses a significant challenge to red kidney beans cultivation, resulting in yield losses and quality deterioration. This study investigates the molecular mechanisms using Tandem Mass Tag (TMT) based proteomics technology to explore how the plant growth-promoting rhizobacterium (PGPR) A-27 enhances the resistance of red kidney beans against SCN. The results revealed that out of 1,374 differentially expressed proteins (DEPs) in the red kidney beans roots, 734 DEPs were upregulated and 640 DEPs were downregulated in the A-27 + J2 J2 treatment group. KEGG analysis revealed that 14 DEPs were involved in the α-LeA metabolic pathway, crucial for the biosynthesis of jasmonic acid (JA) in plants. Quantitative real-time PCR (qRT-PCR) confirmed the upregulation of 4 key genes (, , ) in the JA biosynthesis pathway, while enzyme-linked immunosorbent assay (ELISA) demonstrated a significant increase in JA content in the roots. The study demonstrates that A-27 stimulates induced systemic resistance (ISR) in red kidney beans, and induce JA biosynthesis by regulating the expression of key enzymes in the α-LeA metabolic pathway. This enhances the plant's defense against SCN, providing a theoretical foundation for the potential use of A-27 as a biocontrol agent for managing SCN in leguminous crops.
大豆胞囊线虫（SCN）对红芸豆种植构成重大挑战，导致产量损失和品质下降。本研究利用基于串联质谱标签（TMT）的蛋白质组学技术研究分子机制，以探索植物促生根际细菌（PGPR）A-27如何增强红芸豆对SCN的抗性。结果显示，在红芸豆根部的1374个差异表达蛋白（DEP）中，A-27 + J2 J2处理组中有734个DEP上调，640个DEP下调。KEGG分析表明，14个DEP参与α-LeA代谢途径，这对植物中茉莉酸（JA）的生物合成至关重要。实时定量聚合酶链反应（qRT-PCR）证实了JA生物合成途径中4个关键基因（，，）的上调，而酶联免疫吸附测定（ELISA）表明根部JA含量显著增加。该研究表明，A-27刺激红芸豆中的诱导系统抗性（ISR），并通过调节α-LeA代谢途径中关键酶的表达来诱导JA生物合成。这增强了植物对SCN的防御能力，为将A-27作为一种生物防治剂用于管理豆科作物中的SCN提供了理论基础。
