环糊精和树枝状聚合物修饰的氧化石墨烯作为载体用于传递选定的化疗药物和光敏剂。
Cyclodextrin- and dendrimer-conjugated graphene oxide as a nanocarrier for the delivery of selected chemotherapeutic and photosensitizing agents.
机构信息
Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Section 4, Keelung Road, Taipei 10607, Taiwan, ROC.
Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Section 4, Keelung Road, Taipei 10607, Taiwan, ROC.
出版信息
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:307-315. doi: 10.1016/j.msec.2018.04.020. Epub 2018 Apr 12.
In this study, nanohybrid materials consisting of graphene oxide (GO), β‑cyclodextrin (CD) and poly(amido amine) dendrimer (DEN) were successfully prepared by covalent bonding. GO-CD and GO-CD-DEN were found to be potential nanocarriers for anticancer drugs including chemotherapeutics (doxorubicin (DOX), camptothecin (CPT)) and photosensitizer (protoporphyrin IX (PpIX)). GO-CD possessed 1.2 times higher DOX-loading capacity than GO due to inclusion of additional DOX to the CD. The drug loading on GO-CD-DEN increased in the order: DOX < PpIX < CPT. Enhanced cytotoxicity of DOX and CPT and also the photocytotoxicity of PpIX were observed when the drugs were loaded in GO-CD and GO-CD-DEN. Functionalization of GO with CD and CD-DEN increased the uptake in cancer cells, and both GO-CD and GO-CD-DEN nanohybrids remained in the cytoplasm and were not uptaken into the nucleus, as shown in the flow cytometry and high-content screening study.
在这项研究中，通过共价键成功制备了由氧化石墨烯（GO）、β-环糊精（CD）和聚酰胺-胺树枝状大分子（DEN）组成的纳米杂化材料。GO-CD 和 GO-CD-DEN 被发现是抗癌药物的潜在纳米载体，包括化疗药物（阿霉素（DOX）、喜树碱（CPT））和光敏剂（原卟啉 IX（PpIX））。由于 CD 中包含了额外的 DOX，GO-CD 的 DOX 载药能力比 GO 高 1.2 倍。GO-CD-DEN 的药物载药量顺序为：DOX < PpIX < CPT。当药物负载到 GO-CD 和 GO-CD-DEN 中时，观察到 DOX 和 CPT 的细胞毒性增强，以及 PpIX 的光细胞毒性。CD 和 CD-DEN 对 GO 的功能化增加了癌细胞的摄取，并且如流式细胞术和高内涵筛选研究所示，GO-CD 和 GO-CD-DEN 纳米杂化物都留在细胞质中，而不会被摄取到细胞核中。
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