Kaur Inderpreet, Gaba Sonal, Kaur Sukhraj, Kumar Rajeev, Chawla Jyoti
Department of Chemistry, Center for Advanced Studies, Guru Nanak Dev University, Amritsar, India E-mail:
Department of Microbiology, Guru Nanak Dev University, Amritsar, India.
Water Sci Technol. 2018 May;77(9-10):2204-2212. doi: 10.2166/wst.2018.123.
A spectrophotometric method based on diazotization of aniline with triclosan has been developed for the determination of triclosan in water samples. The diazotization process involves two steps: (1) reaction of aniline with sodium nitrite in an acidic medium to form diazonium ion and (2) reaction of diazonium ion with triclosan to form a yellowish-orange azo compound in an alkaline medium. The resulting yellowish-orange product has a maximum absorption at 352 nm which allows the determination of triclosan in aqueous solution in the linear concentration range of 0.1-3.0 μM with R = 0.998. The concentration of hydrochloric acid, sodium nitrite, and aniline was optimized for diazotization reaction to achieve good spectrophotometric determination of triclosan. The optimization of experimental conditions for spectrophotometric determination of triclosan in terms of concentration of sodium nitrite, hydrogen chloride and aniline was also carried out by using Box-Behnken design of response surface methodology and results obtained were in agreement with the experimentally optimized values. The proposed method was then successfully applied for analyses of triclosan content in water samples.
已开发出一种基于苯胺与三氯生重氮化反应的分光光度法，用于测定水样中的三氯生。重氮化过程包括两个步骤：（1）苯胺在酸性介质中与亚硝酸钠反应形成重氮离子；（2）重氮离子在碱性介质中与三氯生反应形成橙黄色偶氮化合物。所得橙黄色产物在352 nm处有最大吸收，这使得能够在0.1 - 3.0 μM的线性浓度范围内测定水溶液中的三氯生，相关系数R = 0.998。对重氮化反应中盐酸、亚硝酸钠和苯胺的浓度进行了优化，以实现对三氯生的良好分光光度测定。还采用响应面法的Box - Behnken设计对分光光度法测定三氯生时亚硝酸钠、氯化氢和苯胺浓度的实验条件进行了优化，所得结果与实验优化值一致。该方法随后成功应用于水样中三氯生含量的分析。
