Philippe Charlotte, Gregoir Arnout F, Thoré Eli S J, De Boeck Gudrun, Brendonck Luc, Pinceel Tom
Animal Ecology, Global Change and Sustainable Development, University of Leuven; Systemic Physiological and Ecotoxicological Research, University of Antwerp;
Animal Ecology, Global Change and Sustainable Development, University of Leuven.
J Vis Exp. 2018 Apr 24(134):57308. doi: 10.3791/57308.
The killifish Nothobranchius furzeri is an emerging model organism in the field of ecotoxicology and its applicability in acute and chronic ecotoxicity testing has been demonstrated. Overall, the sensitivity of the species to toxic compounds is in the range with, or higher than, that of other model species. This work describes protocols for acute, chronic, and multigenerational bioassays of single and combined stressor effects on N. furzeri. Due to its short maturation time and life-cycle, this vertebrate model allows the study of endpoints such as maturation time and fecundity within four months. Transgenerational full life-cycle exposure trials can be performed in as little as 8 months. Since this species produces eggs that are drought-resistant and remain viable for years, the on-site culture of the species is not needed but individuals can be recruited when required. The protocols are designed to measure life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
鳉鱼（Nothobranchius furzeri）是生态毒理学领域新兴的模式生物，其在急性和慢性生态毒性测试中的适用性已得到证实。总体而言，该物种对有毒化合物的敏感性与其他模式物种相当或更高。这项工作描述了对N. furzeri单一和复合应激源影响进行急性、慢性和多代生物测定的方案。由于其成熟时间和生命周期较短，这个脊椎动物模型能够在四个月内研究诸如成熟时间和繁殖力等终点指标。跨代全生命周期暴露试验最短可在8个月内完成。由于该物种产生的卵具有抗旱性且能存活数年，因此无需在现场养殖该物种，但可在需要时招募个体。这些方案旨在测量生活史特征（死亡率、生长、繁殖力、体重）和临界热最大值。
