Janssen J J, van de Ven W J, van Groningen-Luyben W A, Roosien J, Vlak J M, de Grip W J
Department of Biochemistry, University of Nijmegen, The Netherlands.
Mol Biol Rep. 1988;13(2):65-71. doi: 10.1007/BF00539052.
In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1 x 10(6) insect cells produced up to 3 micrograms opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.
使用杆状病毒表达载体系统实现了编码牛视蛋白的cDNA的体外表达。合成了全长视蛋白，它能被针对牛视紫红质产生的多克隆和单克隆抗血清识别。用重组病毒感染后，1×10⁶个昆虫细胞可产生多达3微克的视蛋白。将体外合成的视蛋白与11-顺式视黄醛一起温育，产生了一种对羟胺稳定的光敏色素。
