Petit Vanessa
CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France.
Université Paris-Diderot, Paris, France.
Methods Mol Biol. 2018;1784:93-98. doi: 10.1007/978-1-4939-7837-3_9.
Macrophages are one of the most abundant leucocytes in the intestinal mucosa where they are essential for maintaining homeostasis. However they are also implicated in the pathogenesis of disorders such as inflammatory bowel disease (IBD), offering potential targets for novel therapies.Tissue macrophages are a heterogeneous population of immune cells that fulfill tissue-specific and niche-specific functions. These unique phenotypes likely reflect the heterogeneity of tissue macrophage origins and influence the tissue environment in which they reside. Here we describe how we can characterize and isolate the colonic macrophages.
巨噬细胞是肠道黏膜中最丰富的白细胞之一，对维持体内平衡至关重要。然而，它们也与诸如炎症性肠病（IBD）等疾病的发病机制有关，为新型疗法提供了潜在靶点。组织巨噬细胞是一类异质性免疫细胞群体，具有组织特异性和特定微环境特异性功能。这些独特的表型可能反映了组织巨噬细胞来源的异质性，并影响它们所驻留的组织环境。在这里，我们描述了如何对结肠巨噬细胞进行表征和分离。
