Rupasinghe Thusitha W T, Roessner Ute
Metabolomics Australia, School of BioSciences, The University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol. 2018;1778:125-135. doi: 10.1007/978-1-4939-7819-9_9.
Lipids are defined as hydrophobic or amphipathic small molecules which consist of a number of structurally and functionally distinct molecules that span from nonpolar to neutral to polar compounds. Lipidomics is the comprehensive analysis of all lipids in a biological system. Changes in lipid metabolism and composition, as well as of distinct lipid species have been linked with altered plant growth, development, and responses to environmental stresses including salinity. Recently, improved liquid chromatography mass spectrometry (LC-MS)-based techniques have provided the rapid expansion of lipidomics research. Sample preparation and lipid extraction are important steps in lipidomics, and this chapter describes important considerations in lipid monophasic and biphasic extractions from plant tissues prior to untargeted plant lipidomics approaches with LC-MS.
脂质被定义为疏水或两亲性小分子，由许多结构和功能各异的分子组成，涵盖从非极性到中性再到极性的化合物。脂质组学是对生物系统中所有脂质进行的全面分析。脂质代谢和组成的变化，以及不同脂质种类的变化，都与植物生长、发育以及对包括盐度在内的环境胁迫的反应改变有关。最近，基于液相色谱-质谱联用（LC-MS）的改进技术推动了脂质组学研究的迅速发展。样品制备和脂质提取是脂质组学中的重要步骤，本章介绍了在采用LC-MS进行非靶向植物脂质组学分析之前，从植物组织中进行脂质单相和双相提取时的重要注意事项。
