使用超高效液相色谱-四极杆飞行时间质谱联用技术的植物脂质组学
Plant Lipidomics Using UPLC-QTOF-MS.
作者信息
Okazaki Yozo, Saito Kazuki
机构信息
Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Graduate School of Bioresources, Mie University, Tsu, Mie, Japan.
出版信息
Methods Mol Biol. 2018;1778:157-169. doi: 10.1007/978-1-4939-7819-9_11.
Lipids are primary metabolites used for energy storage, signal transduction, and pigmentation, besides being common membrane components. Considering the importance of comprehensive lipid analysis (lipidomics) for a detailed understanding of cellular metabolic states, this chapter introduces liquid chromatography-quadrupole time-of-flight mass spectrometry as a powerful untargeted plant lipidomics technique.
脂质是主要的代谢产物，除了作为常见的膜成分外，还用于能量储存、信号转导和色素沉着。鉴于全面脂质分析（脂质组学）对于详细了解细胞代谢状态的重要性，本章介绍液相色谱-四极杆飞行时间质谱法，这是一种强大的非靶向植物脂质组学技术。
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