Lu Yi, Eiriksson Finnur Freyr, Thorsteinsdóttir Margrét, Simonsen Henrik Toft
Department of Biotechnology and Biomedicine Technical University of Denmark Kongens Lyngby Denmark.
ArcticMass Reykjavik Iceland.
Plant Environ Interact. 2022 Dec 22;3(6):254-263. doi: 10.1002/pei3.10095. eCollection 2022 Dec.
Bryophytes, which lack lignin for protection, support themselves in harsh environments by producing various chemicals. In response to cold stress, lipids play a crucial role in cell adaptation and energy storage. Specifically, bryophytes survive at low temperatures by producing very long-chain polyunsaturated fatty acids (vl-PUFAs). The in-depth understanding of the lipid response to cold stress of bryophytes was studied by performing lipid profiling using ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS). Two moss species ( and ) cultivated at 23°C and at 10°C were included in this study. Relative quantitative lipid concentrations were compared and the potential lipid biomarkers were identified by multivariate statistical analysis in each species. In , it was observed that the phospholipids and glycolipids increased under cold stress, while storage lipids decreased. The accumulation of the lipids with high unsaturation degrees mostly appears in phospholipids and glycolipids for both mosses. The results also indicate that two unusual lipid classes in plants, sulfonolipids and phosphatidylmethanol are biosynthesized by the bryophytes. This has not been seen previously and show that bryophytes have a very diverse chemistry and substantially different from other plant groups.
苔藓植物缺乏木质素保护，通过产生各种化学物质在恶劣环境中维持自身生存。在应对冷胁迫时，脂质在细胞适应和能量储存中起着关键作用。具体而言，苔藓植物通过产生极长链多不饱和脂肪酸（vl-PUFAs）在低温下存活。通过使用超高效液相色谱-四极杆飞行时间质谱（UHPLC-QTOF-MS）进行脂质谱分析，对苔藓植物脂质对冷胁迫的响应进行了深入研究。本研究纳入了在23°C和10°C下培养的两种苔藓（ 和 ）。比较了相对定量的脂质浓度，并通过多变量统计分析确定了每个物种中的潜在脂质生物标志物。在 中，观察到冷胁迫下磷脂和糖脂增加，而储存脂质减少。两种苔藓中，高度不饱和脂质的积累大多出现在磷脂和糖脂中。结果还表明，植物中的两种特殊脂质类别，即磺脂和磷脂酰甲醇是由苔藓植物生物合成的。这在以前未曾见过，表明苔藓植物具有非常多样的化学组成，与其他植物类群有很大不同。
