Aix Marseille Univ , CNRS, Centrale Marseille, Institut Fresnel , Marseille , France.
Département de Physique , Ecole Normale Supérieure/PSL Research University, CNRS , 24 rue Lhomond , 75005 Paris , France.
Anal Chem. 2018 Jun 19;90(12):7197-7203. doi: 10.1021/acs.analchem.7b05303. Epub 2018 May 25.
We experimentally implement a compressive Raman technology (CRT) that incorporates chemometric analysis directly into the spectrometer hardware by means of a digital micromirror device (DMD). The DMD is a programmable optical filter on which optimized binary filters are displayed. The latter are generated with an algorithm based on the Cramer-Rao lower bound. We compared the developed CRT microspectrometer with two conventional state-of-the-art Raman hyperspectral imaging systems on samples mimicking microcalcifications relevant for breast cancer diagnosis. The CRT limit of detection significantly improves, when compared to the CCD based system, and CRT ultimately allows 100× and 10× faster acquisition speeds than the CCD- and EMCCD-based systems, respectively.
我们通过数字微镜器件（DMD）将化学计量分析直接纳入光谱仪硬件，实验性地实现了压缩拉曼技术（CRT）。DMD 是一种可编程光学滤波器，可在其上显示优化的二进制滤波器。后者是使用基于克拉美罗下限的算法生成的。我们将开发的 CRT 微光谱仪与两个模拟乳腺癌诊断相关的微钙化的常规最先进的拉曼高光谱成像系统进行了比较。与基于 CCD 的系统相比，CRT 的检测限有了显著提高，并且 CRT 最终允许比基于 CCD 和 EMCCD 的系统分别快 100 倍和 10 倍的采集速度。
