Department of Chemistry, Universität Konstanz, 78464 Konstanz, Germany.
J Phys Chem B. 2023 Jul 13;127(27):6029-6037. doi: 10.1021/acs.jpcb.3c01407. Epub 2023 Jul 5.
Stimulated Raman Scattering microscopy is an important imaging technique. Its broader application, however, is hampered by its comparatively low sensitivity. Using organic fluorophores, it has recently been demonstrated that, similar to spontaneous Raman microscopy, the sensitivity of stimulated Raman microscopy is increased by orders of magnitudes if electronic preresonances are exploited. In this Article, we show that this approach also works with low quantum yield chromophores. We investigate the relevant photophysics and discuss the background arising from preresonant excitation conditions. Applications of preresonant stimulated Raman scattering microscopy for the imaging of weakly fluorescing labels in fixed and live cells are demonstrated.
受激拉曼散射显微镜是一种重要的成像技术。然而，由于其相对较低的灵敏度，其应用受到了限制。最近，利用有机荧光团已经证明，类似于自发拉曼显微镜，如果利用电子预共振，受激拉曼显微镜的灵敏度可以提高几个数量级。在本文中，我们表明这种方法也适用于量子产率低的发色团。我们研究了相关的光物理，并讨论了来自预共振激发条件的背景。展示了预共振受激拉曼散射显微镜在固定和活细胞中弱荧光标记成像中的应用。
