1 Department of Ultrasonography, the Affiliated Hongqi Hospital of Mudanjiang Medical University, Heilongjiang Province 157011, China.
2 Office of Student Affairs, School of Basic Medical Sciences of Mudanjiang Medical University, Heilongjiang Province 157011, China.
Exp Biol Med (Maywood). 2018 May;243(9):749-753. doi: 10.1177/1535370218775042.
The current study aimed to investigate the relations of three single nucleotide polymorphisms of matrix metalloproteinase-9 gene, and single nucleotide polymorphisms-smoking interaction to subarachnoid hemorrhage risk. The optimal pattern of the interaction among single nucleotide polymorphisms and smoking was selected by generalized multifactor dimensionality reduction. The association between the three single nucleotide polymorphisms within the matrix metalloproteinase-9 gene was analyzed by logistic regression test. As well as genetic risk of subarachnoid hemorrhage interactions with smoking, the risk of subarachnoid hemorrhage in carriers with the rs3918242 (T) was significantly higher than in carriers carrying CC (genotype: CT + TT vs. CC), adjusted OR (95% CI) = 1.58 (1.25-2.03), and in carriers carrying rs17576- (genotype: AG + GG vs. AA), adjust OR (95% CI) = 1.62 (1.19-2.13). However, after adjusting for covariates, we did not find any direct association between rs17577 and subarachnoid hemorrhage risk. The generalized multifactor dimensionality reduction model shows a potential relation between rs3918242 and smoking risk for subarachnoid hemorrhage ( P = 0.0010). After covariates adjustment, current smokers with rs3918242-CT or TT genotype, compared to never-smokers with rs3918242-CC genotype, OR (95% CI) = 2.57 (1.74-3.46), have a higher subarachnoid hemorrhage risk. Our study showed that the rs3918242 (T) and rs17576 (G), the cross reaction between rs3918242 and smoking increased the risk of subarachnoid hemorrhage. Impact statement Matrix metalloproteinase-9 ( MMP-9) is a possible candidate gene for some diseases, including metabolic syndrome, stroke, coronary artery disease (CAD). But to date, limited data focused on the relationship between MMP-9 gene SNPs and SAH susceptibility. The purpose of this study was to evaluate SNPs of MMP-9 gene and their interaction with environmental factors with SAH risk based on a Chinese population.
本研究旨在探讨基质金属蛋白酶-9 基因的三个单核苷酸多态性以及单核苷酸多态性-吸烟相互作用与蛛网膜下腔出血风险的关系。通过广义多因素降维法选择单核苷酸多态性和吸烟相互作用的最佳模式。通过 logistic 回归检验分析基质金属蛋白酶-9 基因内三个单核苷酸多态性之间的关联。以及遗传风险与吸烟的相互作用，rs3918242（T）携带者的蛛网膜下腔出血风险明显高于 CC 携带者（基因型：CT+TT 与 CC），调整后的比值比（95%CI）=1.58（1.25-2.03），rs17576-（基因型：AG+GG 与 AA）携带者，调整后的比值比（95%CI）=1.62（1.19-2.13）。然而，在调整了协变量后，我们没有发现 rs17577 与蛛网膜下腔出血风险之间存在直接关联。广义多因素降维模型显示 rs3918242 与吸烟对蛛网膜下腔出血的风险之间存在潜在关系（P=0.0010）。调整协变量后，与 rs3918242-CC 基因型的从不吸烟者相比，rs3918242-CT 或 TT 基因型的当前吸烟者的 OR（95%CI）=2.57（1.74-3.46），蛛网膜下腔出血风险更高。我们的研究表明，rs3918242（T）和 rs17576（G），rs3918242 与吸烟的交叉反应增加了蛛网膜下腔出血的风险。
基质金属蛋白酶-9（MMP-9）是包括代谢综合征、中风、冠心病（CAD）在内的某些疾病的候选基因。但迄今为止，有限的数据集中在 MMP-9 基因 SNPs 与蛛网膜下腔出血易感性之间的关系上。本研究旨在基于中国人群评估 MMP-9 基因 SNPs 及其与环境因素的相互作用与蛛网膜下腔出血风险的关系。
