Yomantas Yurgis A V, Abalakina Elena G, Lobanova Juliya S, Mamontov Victor A, Stoynova Nataliya V, Mashko Sergey V
Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, 117545, Moscow, Russian Federation.
Arch Virol. 2018 Sep;163(9):2565-2568. doi: 10.1007/s00705-018-3867-x. Epub 2018 May 15.
The genomes of two new lytic phages of Corynebacterium glutamicum ATCC 13032, φ673 and φ674, were sequenced and annotated (GenBank: MG324353, MG324354). Electron microscopy studies of both virions revealed that taxonomically they belong to the Siphoviridae family and have a polyhedral head with a width of 50 nm and a non-contractile tail with a length of 250 nm. The genomes of φ673 and φ674 consist of linear double-stranded DNA molecules with lengths of 44,530 bp (G+C = 51.1%) and 43,193 bp (G+C = 50.7%) and identical, protruding, cohesive 3' ends 13 nt in length. The level of identity between the φ673 and φ674 genomes is 85.2%. Two major structural proteins of each virion were separated via SDS-PAGE and identified using peptide mass fingerprinting. Based on bioinformatic analysis, 56 and 54 ORFs were predicted for φ673 and φ674, respectively. Only 20 of the putative gene products of φ673 and 20 of φ674 could be assigned to known functions. Both genomes were divided into functional modules. Nine putative promoters in the φ673 genome and eight in the φ674 genome were predicted. One bidirectional Rho-independent transcription terminator was identified and experimentally confirmed in each phage genome.
对谷氨酸棒杆菌ATCC 13032的两种新型裂解性噬菌体φ673和φ674的基因组进行了测序和注释（GenBank：MG324353，MG324354）。对这两种病毒粒子的电子显微镜研究表明，从分类学角度来看，它们属于长尾噬菌体科，具有一个宽度为50纳米的多面体头部和一条长度为250纳米的非收缩性尾部。φ673和φ674的基因组由线性双链DNA分子组成，长度分别为44,530碱基对（G+C = 51.1%）和43,193碱基对（G+C = 50.7%），并且具有相同的、突出的、粘性的3'末端，长度为13个核苷酸。φ673和φ674基因组之间的同一性水平为85.2%。通过SDS-PAGE分离了每个病毒粒子的两种主要结构蛋白，并使用肽质量指纹图谱进行了鉴定。基于生物信息学分析，分别预测了φ673和φ674的56个和54个开放阅读框。φ673的假定基因产物中只有20个和φ674的20个可以被指定为已知功能。两个基因组都被分为功能模块。在φ673基因组中预测有9个假定启动子，在φ674基因组中预测有8个。在每个噬菌体基因组中鉴定并通过实验证实了一个双向的不依赖Rho的转录终止子。
