Haghighi Saboura, Reza Goli Hamid
Sana Institute of Higher Education, Sari, Mazandaran, Iran.
Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.
AIMS Microbiol. 2022 Apr 25;8(2):153-166. doi: 10.3934/microbiol.2022013. eCollection 2022.
The increased prevalence of β-lactamase is one of the main factors in resistance to β-lactams in . This study aimed to investigate the prevalence of , , and genes in β-lactam-resistant . We collected 100 non-duplicated clinical isolates of and identified them by standard tests. Using disk agar diffusion test, we detected the β-lactam-resistant isolates and extracted the DNAs of the isolates by alkaline lysis method. Then, the prevalence of , and genes were detected by PCR method. The results were assessed by SPSS 21 software and Chi-square test. Out of 100 isolates, 43% were detected as resistant against at least one of the beta-lactams tested. Piperacillin-tazobactam was the most effective antibiotic, while 39% and 37% of the isolates were resistant to aztreonam and meropenem, respectively. A significant relationship was observed between the resistance to tested antibiotics and the presence of , , and genes. Among 43 isolates that were resistant to at least one of the tested β-lactams, 93.02%, 83.72%, and 81.39% of them carried , , and genes, respectively. According to this study and due to high prevalence of β-lactam resistance genes, it is better to check the level of antibiotic resistance and resistance genes for better management of patients with infection caused by this bacterium. Also, high prevalence of class A β-lactamases indicates the significant role of these enzymes in emerging resistance to beta-lactams.
β-内酰胺酶患病率的增加是[具体对象]对β-内酰胺类抗生素耐药的主要因素之一。本研究旨在调查β-内酰胺类抗生素耐药[具体对象]中[相关基因名称1]、[相关基因名称2]和[相关基因名称3]基因的患病率。我们收集了100株非重复的[具体对象]临床分离株，并通过标准试验对其进行鉴定。采用纸片琼脂扩散试验检测β-内酰胺类抗生素耐药分离株，并通过碱裂解法提取分离株的DNA。然后，采用PCR方法检测[相关基因名称1]、[相关基因名称2]和[相关基因名称3]基因的患病率。结果采用SPSS 21软件和卡方检验进行评估。在100株分离株中，43%被检测出对至少一种受试β-内酰胺类抗生素耐药。哌拉西林-他唑巴坦是最有效的抗生素，而39%和37%的分离株分别对氨曲南和美罗培南耐药。观察到受试抗生素耐药性与[相关基因名称1]、[相关基因名称2]和[相关基因名称3]基因的存在之间存在显著相关性。在对至少一种受试β-内酰胺类抗生素耐药的43株分离株中，分别有93.02%、83.72%和81.39%携带[相关基因名称1]、[相关基因名称2]和[相关基因名称3]基因。根据本研究，由于β-内酰胺类抗生素耐药基因的高患病率，最好检查抗生素耐药水平和耐药基因，以便更好地管理由该细菌引起感染的患者。此外，A类β-内酰胺酶的高患病率表明这些酶在β-内酰胺类抗生素新出现的耐药性中起重要作用。
