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在塞尔维亚医院环境中传播的产金属β-内酰胺酶：ST235 和 ST654 克隆的扩展。

Dissemination of Metallo-β-Lactamase-Producing in Serbian Hospital Settings: Expansion of ST235 and ST654 Clones.

机构信息

Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Dr Subotica Starijeg 1, 11000 Belgrade, Serbia.

CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Universidade Católica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.

出版信息

Int J Mol Sci. 2023 Jan 12;24(2):1519. doi: 10.3390/ijms24021519.

DOI:10.3390/ijms24021519
PMID:36675030
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9863560/
Abstract

This nationwide study aimed to investigate the molecular characteristics of metallo-β-lactamase (MBL)-producing in Serbia, underlying resistance mechanisms, the genetic context of detected MBL genes, and the clonal relationship between isolates harboring genes-encoding MBL. Overall, 320/5334 isolates collected from 2018 to 2021 were identified as . Carbapenem-resistant (CRPA) were screened for the presence of , , and , genes whereas MBL-positive isolates were tested for the presence of the , , , , , and . Multilocus sequence typing and phylogenomic analysis were performed for -producing MBL. The majority of the were recovered from the lower respiratory tract (n = 120; 37.5%) and wound specimens (n = 108; 33.75%). CRPA isolates accounted for 43.1% (n = 138) of the tested isolates, 31 out of them being -positive (22.5%). The colistin resistance rate was 0.3%. MLST analysis revealed the occurrence of ST235 (n = 25) and ST654 (n = 6), mostly confined to Serbia. The distribution of beta-lactamase-encoding genes in these isolates suggested clonal dissemination and possible recombination: ST235/, ST235//, ST654/, ST654//, and ST654//. High-risk clones ST235 and ST654 identified for the first time in Serbia, are important vectors of acquired MBL and ESBL and their associated multidrug resistance phenotypes represent a cause for considerable concern.

摘要

这项全国性研究旨在调查塞尔维亚产金属β-内酰胺酶（MBL）的分子特征、耐药机制、检测到的 MBL 基因的遗传背景以及携带 MBL 基因的分离株之间的克隆关系。总的来说，从 2018 年到 2021 年收集的 5334 株分离株中，有 320 株被鉴定为 。对碳青霉烯耐药的 （CRPA）进行了筛选，以确定 是否存在 、 、 和 基因，而 MBL 阳性分离株则进行了 是否存在 、 、 、 、 和 基因的检测。对产 MBL 的 进行了多位点序列分型和系统发育分析。大多数 是从下呼吸道（n = 120；37.5%）和伤口标本（n = 108；33.75%）中回收的。CRPA 分离株占检测分离株的 43.1%（n = 138），其中 31 株为 -阳性（22.5%）。粘菌素耐药率为 0.3%。MLST 分析显示 ST235（n = 25）和 ST654（n = 6）的发生，主要局限于塞尔维亚。这些分离株中β-内酰胺酶编码基因的分布表明了克隆传播和可能的重组：ST235/ 、 ST235// 、 ST654/ 、 ST654// 和 ST654//。首次在塞尔维亚发现的高风险克隆 ST235 和 ST654 是获得性 MBL 和 ESBL 的重要载体，其相关的多药耐药表型引起了极大的关注。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be88/9863560/40ce26e538de/ijms-24-01519-g006.jpg
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