Institute: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, China.
Infinitus Chinese Herbal Immunity Research Centre, Guangzhou, China.
Int Immunopharmacol. 2018 Jul;60:221-227. doi: 10.1016/j.intimp.2018.05.003. Epub 2018 May 14.
Both probiotics and pathogens in the human gut express pathogen-associated molecular patterns (PAMPs) and die with the release of endotoxin and bacterial DNA, which can stimulate our immune system and cause immune reaction. However, it's interesting and fascinating to address why the normal intestinal flora will not generate immunological rejection like the pathogen does. By investigating the changes in cells and molecules relevant to immune tolerance in mice with ceftriaxone-induced dysbacteriosis, our study discovered that both the Evenness indexes and Shannon Wiener index of intestinal flora showed a decrease in dysbacteriosis mice. Moreover, the proportion of αβTCRCD3CD4CD8 cells, CD3γδTCR cells and CD4CD25FoxP3 cells in the Peyer's patches (PPs), mesenteric lymph nodes (MLNs) and spleen (SP) and the level of TGF-β1, IL-2, IL-4 and IL-10 in the serum also changed. Intestinal dysbacteriosis in an asthma murine model resulted in enhancement of immunologic response to the allergen ovalbumin (OVA), which was an agent that aggravates asthma symptoms. In summary, it is integral to maintain a certain amount or variety of intestinal microflora for regulatory T cells to act in averting hypersensitivity.
人体肠道中的益生菌和病原体都表达病原体相关分子模式（PAMPs），并随着内毒素和细菌 DNA 的释放而死亡，这些物质可以刺激我们的免疫系统并引发免疫反应。然而，有趣的是，为什么正常的肠道菌群不会像病原体那样产生免疫排斥。通过研究头孢曲松诱导的肠道菌群失调小鼠中与免疫耐受相关的细胞和分子的变化，我们的研究发现，肠道菌群的均匀度指数和香农威纳指数均在菌群失调小鼠中下降。此外，派尔集合淋巴结（PPs）、肠系膜淋巴结（MLNs）和脾脏（SP）中 αβTCRCD3CD4CD8 细胞、CD3γδTCR 细胞和 CD4CD25FoxP3 细胞的比例以及血清中 TGF-β1、IL-2、IL-4 和 IL-10 的水平也发生了变化。哮喘小鼠模型中的肠道菌群失调导致对过敏原卵清蛋白（OVA）的免疫反应增强，这是加重哮喘症状的因素。总之，维持一定数量或种类的肠道微生物对于调节性 T 细胞发挥作用以避免过敏至关重要。
