Yuan Hai-Bin, Ding Yu-Xiao, Gu Shao-Hua, Sun Liang, Zhu Xiao-Qiang, Liu Hang-Wei, Dhiloo Khalid Hussain, Zhang Yong-Jun, Guo Yu-Yuan
College of Agronomy, Jilin Agricultural University, Changchun, Jilin, 130118, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
PLoS One. 2015 Oct 14;10(10):e0140562. doi: 10.1371/journal.pone.0140562. eCollection 2015.
Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is one of the most important agricultural pests, with broad host range and cryptic feeding habits in China. Chemosensory behavior plays an important role in many crucial stages in the life of A. lucorum, such as the detection of sex pheromone cues during mate pursuit and fragrant odorants during flowering host plant localization. Odorant-binding proteins (OBPs) are involved in the initial biochemical recognition steps in semiochemical perception. In the present study, a transcriptomics-based approach was used to identify potential OBPs in A. lucorum. In total, 38 putative OBP genes were identified, corresponding to 26 'classic' OBPs and 12 'Plus-C' OBPs. Phylogenetic analysis revealed that A. lucorum OBP proteins are more closely related to the OBP proteins of other mirid bugs as the same family OBP clustering together. Quantitative real-time PCR analysis for the first reported 23 AlucOBPs revealed that the expression level of 11 AlucOBP genes were significantly higher in antennae of both sexes than in other tissues. Three of them were male antennae-biased and six were female antennae-biased, suggesting their putative roles in the detection of female sex pheromones and host plant volatiles. In addition, three, four, two and one AlucOBPs had the highest degree of enrichment in the stylet, head, leg, and in abdomen tissues, respectively. Two other OBPs were ubiquitously expressed in the main tissues, including antennae, stylets, heads, legs and wings. Most orthologs had similar expression patterns, strongly indicating that these genes have the same function in olfaction and gustation.
绿盲蝽（Meyer-Dür）（半翅目：盲蝽科）是中国最重要的农业害虫之一，具有广泛的寄主范围和隐匿的取食习性。化学感应行为在绿盲蝽生命中的许多关键阶段发挥着重要作用，例如在求偶过程中检测性信息素线索以及在定位开花寄主植物时检测芳香气味物质。气味结合蛋白（OBPs）参与了信息化学物质感知的初始生化识别步骤。在本研究中，采用基于转录组学的方法来鉴定绿盲蝽中潜在的OBPs。总共鉴定出38个假定的OBP基因，对应于26个“经典”OBPs和12个“Plus-C”OBPs。系统发育分析表明，绿盲蝽OBP蛋白与其他盲蝽科昆虫的OBP蛋白关系更密切，同一科的OBP聚集在一起。对首次报道的23个AlucOBPs进行的定量实时PCR分析表明，11个AlucOBP基因在雌雄触角中的表达水平均显著高于其他组织。其中3个在雄虫触角中偏向表达，6个在雌虫触角中偏向表达，这表明它们在检测雌性性信息素和寄主植物挥发物方面可能发挥的作用。此外，分别有3个、4个、2个和1个AlucOBPs在口针、头部、腿部和腹部组织中具有最高的富集程度。另外两个OBPs在包括触角、口针、头部、腿部和翅膀在内的主要组织中普遍表达。大多数直系同源物具有相似的表达模式，强烈表明这些基因在嗅觉和味觉中具有相同的功能。
