Shams Saeed, Hashemi Ali, Esmkhani Mohammad, Kermani Somaye, Shams Elham, Piccirillo Alessandra
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
BMC Res Notes. 2018 May 18;11(1):314. doi: 10.1186/s13104-018-3406-6.
The emergence of metallo-β-lactamase-producing Enterobacteriaceae is a worldwide health concern. In this study, the first evaluation of MBL genes, bla and bla , in Escherichia coli resistant to imipenem isolated from urine and blood specimens in Qom, Iran is described. Three hundred urine and blood specimens were analysed to detect the presence of E. coli. Resistance to imipenem and other antimicrobials was determined by disk diffusion and MIC. MBL production was screened using CDDT. PCR was also carried out to determine the presence of bla and bla genes in imipenem-resistant isolates.
In total, 160 E. coli isolates were collected from March to May 2016. According to disk diffusion, high-level of resistance (20%) to cefotaxime was observed, whereas the lowest (1%) was detected for tetracycline. In addition, five isolates showed resistance to imipenem with a MIC ≥ 4 µg/mL. CDDT test confirmed that five isolates were MBL-producing strains, but no bla and bla genes were detected. Results of this study show a very low level of resistance to imipenem in our geographical area.
产金属β-内酰胺酶肠杆菌科细菌的出现是一个全球性的健康问题。本研究描述了对从伊朗库姆的尿液和血液标本中分离出的耐亚胺培南大肠杆菌中MBL基因bla 和bla 的首次评估。分析了300份尿液和血液标本以检测大肠杆菌的存在。通过纸片扩散法和MIC测定对亚胺培南和其他抗菌药物的耐药性。使用CDDT筛选MBL的产生。还进行了PCR以确定耐亚胺培南菌株中bla 和bla 基因的存在。
2016年3月至5月共收集了160株大肠杆菌分离株。根据纸片扩散法，观察到对头孢噻肟的高水平耐药（20%），而对四环素的耐药率最低（1%）。此外，5株分离株对亚胺培南耐药，MIC≥4 μg/mL。CDDT试验证实5株分离株为产MBL菌株，但未检测到bla 和bla 基因。本研究结果表明我们所在地区对亚胺培南的耐药水平非常低。
