抗坏血酸通过ROS和AKT/mTOR信号传导抑制间充质干细胞衰老。
Ascorbic acid inhibits senescence in mesenchymal stem cells through ROS and AKT/mTOR signaling.
作者信息
Yang Mengkai, Teng Songsong, Ma Chunhui, Yu Yinxian, Wang Peilin, Yi Chengqing
机构信息
Department of Orthopaedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.
出版信息
Cytotechnology. 2018 Oct;70(5):1301-1313. doi: 10.1007/s10616-018-0220-x. Epub 2018 May 18.
Abstract
Mesenchymal stem cell (MSC) aging seriously affects its function in stem cell transplantation for treatment. Extensive studies have focused on how to inhibit senescence in MSCs. However, the mechanism of senescence in MSC was not clear. In this study, we used D-galactose to induce MSC aging. Then we found that the number of aging cells was increased compared with untreated MSCs. We discovered that ascorbic acid could inhibit the production of reactive oxygen species (ROS) and activation of AKT/mTOR signaling in MSCs caused by D-galactose. Especially, when treated together with a ROS scavenger or AKT inhibitor, the senescent cells were obviously decreased in D-galactose-induced MSCs. Taken together, we identify that ascorbic acid owns the potential to inhibit the senescence of MSCs through ROS and Akt/mTOR signaling. Together, our data supports that ascorbic acid can be used to prevent MSCs from senescence, which can enhance the efficiency of stem cell transplantation in the clinic.
摘要
间充质干细胞（MSC）衰老严重影响其在干细胞移植治疗中的功能。大量研究集中在如何抑制间充质干细胞的衰老。然而，间充质干细胞衰老的机制尚不清楚。在本研究中，我们使用D-半乳糖诱导间充质干细胞衰老。然后我们发现，与未处理的间充质干细胞相比，衰老细胞的数量增加了。我们发现抗坏血酸可以抑制D-半乳糖引起的间充质干细胞中活性氧（ROS）的产生和AKT/mTOR信号通路的激活。特别是，当与ROS清除剂或AKT抑制剂一起处理时，D-半乳糖诱导的间充质干细胞中的衰老细胞明显减少。综上所述，我们确定抗坏血酸具有通过ROS和Akt/mTOR信号通路抑制间充质干细胞衰老的潜力。总之，我们的数据支持抗坏血酸可用于防止间充质干细胞衰老，这可以提高临床干细胞移植的效率。
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