Burgess Richard R
University of Wisconsin-Madison, United States.
Protein Expr Purif. 2018 Oct;150:81-85. doi: 10.1016/j.pep.2018.05.007. Epub 2018 May 17.
Through years of research, teaching, troubleshooting, and editing, I have developed a solid working understanding of many aspects of protein purification and protein biochemistry. I have also found that many researchers do not understand the basics, let alone the pitfalls, of the techniques they use. In the case of size exclusion chromatography (SEC), I summarize below some of the rules of thumb, suggestions, limitations and troubleshooting I have found most useful in helping my students and colleagues carry out successful SEC.
通过多年的研究、教学、故障排除和编辑工作，我对蛋白质纯化和蛋白质生物化学的许多方面形成了扎实的实际理解。我还发现，许多研究人员并不了解他们所使用技术的基础知识，更不用说其中的陷阱了。就尺寸排阻色谱法（SEC）而言，我在下面总结了一些经验法则、建议、局限性以及故障排除方法，这些在帮助我的学生和同事成功进行SEC时最为有用。
