Gelman Daniel, Beyth Shaul, Lerer Vanda, Adler Karen, Poradosu-Cohen Ronit, Coppenhagen-Glazer Shunit, Hazan Ronen
Institute of Dental Sciences, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Hadassah Campus, P.O.B 12272, Jerusalem, 91120, Israel; The Military Track of Medicine, Faculty of Medicine, The Hebrew University - Hadassah School of Medicine, Hadassah Campus, P.O.B 12272, Jerusalem, 91120, Israel.
Orthopedic Surgery Department, Hadassah Medical Center, Hadassah University Hospital, P.O.B 12000, Jerusalem, 91120, Israel.
Res Microbiol. 2018 Nov;169(9):531-539. doi: 10.1016/j.resmic.2018.04.008. Epub 2018 May 16.
Clinical applications of bacteriophage therapy have been recently gathering significant attention worldwide, used mostly as rescue therapy in cases of near-fatal antibiotic failure. Thus, clinically relevant in-vivo models presenting both short- and long-term implications of phage therapy given as rescue treatment for fulminant infections are of highest importance. In this study, a cocktail consisting of two lytic bacteriophages was used to evaluate the therapeutic efficacy of phage therapy as a rescue treatment for severe septic peritonitis in a mouse model. We established that a single injection of the bacteriophage cocktail was sufficient to completely reverse a 100% mortality trend caused by Vancomycin-Resistant Enterococcus faecalis, with significant improvement in both the clinical state and laboratory test results, and without harmful effects on the microbiome. The combination of bacteriophages with a suboptimal antibiotic regimen imparts an additional beneficial effect on the treatment success.
噬菌体疗法的临床应用最近在全球范围内受到了极大关注，主要用于在近乎致命的抗生素治疗失败的情况下作为挽救疗法。因此，对于作为暴发性感染挽救治疗的噬菌体疗法的短期和长期影响的临床相关体内模型至关重要。在本研究中，使用由两种裂解性噬菌体组成的鸡尾酒制剂来评估噬菌体疗法作为小鼠模型中严重脓毒症性腹膜炎挽救治疗的疗效。我们确定，单次注射噬菌体鸡尾酒足以完全扭转由耐万古霉素粪肠球菌引起的100%死亡率趋势，临床状态和实验室检查结果均有显著改善，且对微生物群没有有害影响。噬菌体与次优抗生素方案的联合使用对治疗成功具有额外的有益作用。
