Casares-Crespo L, Fernández-Serrano P, Viudes-de-Castro M P
Centro de Investigación y Tecnología Animal-Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain.
Centro de Investigación y Tecnología Animal-Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain.
Theriogenology. 2018 Aug;116:49-52. doi: 10.1016/j.theriogenology.2018.05.008. Epub 2018 May 16.
The present study was designed to prove new rabbit insemination extenders containing aminopeptidase inhibitors (AMIs) with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. In addition, different hormone concentrations were tested in these extenders, evaluating their in vivo effect on rabbit reproductive performance after artificial insemination. A total of 911 females were inseminated with semen diluted with the four experimental extenders (C4 group: 4 μg buserelin/doe in control medium (Tris-citric acid-glucose supplemented with bestatin 10 μM and EDTA 20 mM), C5 group: 5 μg of buserelin/doe in control medium, Q4 group: 4 μg of buserelin/doe into CS-DS nanoparticles in control medium, Q5 group: 5 μg of busereline/doe into CS-DS nanoparticles in control medium). Results showed that fertility was significantly lower in C4 group compared to C5, Q5 and Q4 groups (0.7 versus 0.85, 0.85 and 0.82, respectively). On the contrary, prolificacy was similar in the four experimental groups studied (P > 0.05). We conclude that the CS-DS nanoparticles prepared by a coacervation process as carrier for buserelin acetate allows reducing the concentration of hormone used in extenders supplemented with bestatin and EDTA without affecting the fertility and prolificacy of rabbit females.
本研究旨在验证新型兔精液稀释剂，其含有氨肽酶抑制剂（AMIs），并添加或不添加包裹促性腺激素释放激素（GnRH）类似物的壳聚糖（CS）-硫酸葡聚糖（DS）纳米颗粒。此外，还在这些稀释剂中测试了不同的激素浓度，评估其在人工授精后对兔繁殖性能的体内影响。共有911只雌性兔子接受人工授精，精液用四种实验性稀释剂稀释（C4组：在对照培养基（添加10μM贝司他汀和20mM乙二胺四乙酸的柠檬酸-葡萄糖-Tris）中每只母兔使用4μg布舍瑞林；C5组：在对照培养基中每只母兔使用5μg布舍瑞林；Q4组：在对照培养基中每只母兔将4μg布舍瑞林加入CS-DS纳米颗粒中；Q5组：在对照培养基中每只母兔将5μg布舍瑞林加入CS-DS纳米颗粒中）。结果显示，与C5、Q5和Q4组相比，C4组的受胎率显著降低（分别为0.7、0.85、0.85和0.82）。相反，在所研究的四个实验组中，产仔数相似（P>0.05）。我们得出结论，通过凝聚法制备的CS-DS纳米颗粒作为醋酸布舍瑞林的载体，可降低在添加贝司他汀和乙二胺四乙酸的稀释剂中使用的激素浓度，而不影响兔雌性的受胎率和产仔数。
