Institute of Biology Leiden, Leiden University, The Netherlands.
Institute of Biology Leiden, Leiden University, The Netherlands.
Trends Biotechnol. 2018 Oct;36(10):1025-1039. doi: 10.1016/j.tibtech.2018.04.007. Epub 2018 May 16.
The challenge of sustainably producing highly valuable chemical compounds requires specialized microbial cell factories because many of these compounds can be toxic to microbial hosts. Therefore, solvent-tolerant bacteria are promising production hosts because of their intrinsic tolerance towards these compounds. Recent studies have helped to elucidate the molecular mechanisms involved in solvent tolerance. Advances in synthetic biological tools will enable further development of streamlined solvent-tolerant production hosts and the transfer of solvent-tolerant traits to established industrial strains. In this review, we outline challenges and opportunities to implement solvent tolerance in bacteria as a desired trait for industrial biotechnology.
可持续生产高价值化合物的挑战需要专门的微生物细胞工厂，因为许多这些化合物对微生物宿主具有毒性。因此，耐溶剂细菌是很有前途的生产宿主，因为它们对这些化合物具有内在的耐受性。最近的研究有助于阐明溶剂耐受性涉及的分子机制。合成生物学工具的进步将使简化的耐溶剂生产宿主的进一步发展和耐溶剂特性转移到现有的工业菌株成为可能。在这篇综述中，我们概述了在细菌中实施溶剂耐受性作为工业生物技术的理想特性所面临的挑战和机遇。
