Cárdenas Espinosa María José, Schmidgall Tabea, Pohl Jessica, Wagner Georg, Wynands Benedikt, Wierckx Nick, Heipieper Hermann J, Eberlein Christian
Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research-UFZ, 04318 Leipzig, Germany.
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52428 Jülich, Germany.
Microorganisms. 2023 Mar 25;11(4):837. doi: 10.3390/microorganisms11040837.
Organic olvent-tolerant strains of the Gram-negative bacterial genus are discussed as potential biocatalysts for the biotechnological production of various chemicals. However, many current strains with the highest tolerance are belonging to the species and are classified as biosafety level 2 strains, which makes them uninteresting for the biotechnological industry. Therefore, it is necessary to identify other biosafety level 1 strains with high tolerance towards solvents and other forms of stress, which are suitable for establishing production platforms of biotechnological processes. In order to exploit the native potential of as a microbial cell factory, the biosafety level 1 strain VLB120 and its genome-reduced (GRC) variants as well as the plastic-degrading strain TDA1 were assessed regarding their tolerance towards different -alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol). Toxicity of the solvents was investigated by their effects on bacterial growth rates given as the EC50 concentrations. Hereby, both toxicities as well as the adaptive responses of GRC3 and TDA1 showed EC50 values up to two-fold higher than those previously detected for DOT-T1E (biosafety level 2), one of the best described solvent-tolerant bacteria. Furthermore, in two-phase solvent systems, all the evaluated strains were adapted to 1-decanol as a second organic phase (i.e., OD was at least 0.5 after 24 h of incubation with 1% () 1-decanol), which shows the potential use of these strains as platforms for the bio-production of a wide variety of chemicals at industrial level.
革兰氏阴性细菌属的耐有机溶剂菌株被视为生物技术生产各种化学品的潜在生物催化剂。然而，目前许多耐受性最高的菌株属于该物种，被归类为生物安全2级菌株，这使得它们对生物技术产业来说缺乏吸引力。因此，有必要鉴定出其他对溶剂和其他形式压力具有高耐受性的生物安全1级菌株，这些菌株适合用于建立生物技术过程的生产平台。为了挖掘作为微生物细胞工厂的天然潜力，对生物安全1级菌株VLB120及其基因组精简的（GRC）变体以及塑料降解菌株TDA1对不同链烷醇（1-丁醇、1-己醇、1-辛醇、1-癸醇）的耐受性进行了评估。通过溶剂对细菌生长速率的影响（以EC50浓度表示）来研究溶剂的毒性。据此，GRC3和TDA1的毒性以及适应性反应的EC50值比之前检测到的耐受性最强的溶剂耐受细菌之一DOT-T1E（生物安全2级）高出两倍。此外，在两相溶剂系统中，所有评估菌株都适应了1-癸醇作为第二有机相（即与1%（体积分数）1-癸醇孵育24小时后OD至少为0.5），这表明这些菌株有潜力作为工业规模生物生产各种化学品的平台。
