Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China.
J Invertebr Pathol. 2018 Jun;155:55-63. doi: 10.1016/j.jip.2018.05.005. Epub 2018 May 17.
Spodoptera exigua (Lepidoptera: Noctuidae) larvae were used to analyze the regulation of lipid, protein and carbohydrate metabolism in host larvae infected with the Heliothis virescens ascovirus 3h (HvAV-3h). Using histological sections, significant pathological changes were found in the fat bodies of infected larvae from 24 h to 72 h post-infection (hpi). The lipid and protein contents of the infected larvae were significantly higher than those of the uninfected larvae, while the carbohydrate content of the infected larvae was significantly lower than that of the mock-infected larvae. The selected primary metabolite metabolism-associated genes showed different expression patterns. Further co-relationship analysis of the gene expression level and content changes of primary metabolites indicated the following: the acyl-CoA dehydrogenase and fatty acid synthase genes were closely associated with lipid metabolism, and the hexokinase and the glycogen synthase gene expression levels were related to carbohydrate metabolism, while the aminopeptidase N and the protein disulfide isomerase gene expression levels were not correlated with protein metabolism. These results indicate that the HvAV-3h virus stimulates host larval lipid and protein syntheses and inhibits carbohydrate synthesis.
甜菜夜蛾（鳞翅目：夜蛾科）幼虫被用于分析感染 Heliothis virescens 虹彩病毒 3h（HvAV-3h）的宿主幼虫中脂质、蛋白质和碳水化合物代谢的调节。通过组织学切片，发现感染幼虫的脂肪体在感染后 24 至 72 小时（hpi）出现明显的病理变化。感染幼虫的脂质和蛋白质含量明显高于未感染幼虫，而感染幼虫的碳水化合物含量明显低于假感染幼虫。选择的初级代谢物代谢相关基因表现出不同的表达模式。初级代谢物基因表达水平和含量变化的进一步共同关系分析表明：酰基辅酶 A 脱氢酶和脂肪酸合酶基因与脂质代谢密切相关，而己糖激酶和糖原合酶基因的表达水平与碳水化合物代谢有关，而氨肽酶 N 和蛋白质二硫键异构酶基因的表达水平与蛋白质代谢无关。这些结果表明，HvAV-3h 病毒刺激宿主幼虫的脂质和蛋白质合成，并抑制碳水化合物的合成。
