Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, 410128, P. R. China.
College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, P. R. China.
Sci Rep. 2018 Mar 29;8(1):5367. doi: 10.1038/s41598-018-23715-6.
Heliothis virescens ascovirus 3 h (HvAV-3h), a dsDNA insect virus, belonging to the family Ascoviridae, can infect caterpillars of several Noctuidae species by ovipositing parasitoid wasps. In order to provide a comprehensive overview of the interactive responses of host larvae after infection by the ascovirus, a transcriptome analysis of Spodoptera exigua to HvAV-3h was conducted from 6 to 168 hours post infection (hpi). Approximately 101.64 Gb of RNA sequencing (RNA-seq) data obtained from infected and uninfected S. exigua larvae were used to perform a de novo transcriptome assembly, which generated approximately 62,258 S. exigua unigenes. Using differential gene expression analysis, it was determined that the majority of host transcripts were down-regulated beginning at 6 hpi and continuing throughout the infection period, although there was an increase in up-regulated unigene number during the 12 to 72 hpi stage. It is noteworthy that the most abundantly enriched pathways in KEGG annotation were Metabolism terms, indicating that the host larval metabolic mechanisms were highly influenced post HvAV-3h infection. In addition, the host cuticle protein encoding unigenes were highly down-regulated in most of the situations, suggesting that the host larval cuticle synthesis were inhibited by the viral infection.
棉铃虫野油菜黄单胞病毒 3 小时（HvAV-3h），一种双链 DNA 昆虫病毒，属于虹彩病毒科，能够通过产卵寄生蜂感染几种夜蛾科昆虫的幼虫。为了全面了解宿主幼虫在感染虹彩病毒后的交互反应，对感染 HvAV-3h 的甜菜夜蛾进行了转录组分析，从感染后 6 至 168 小时（hpi）。大约 101.64Gb 的 RNA 测序（RNA-seq）数据来自感染和未感染的甜菜夜蛾幼虫，用于进行从头转录组组装，生成了大约 62258 个甜菜夜蛾 unigenes。通过差异基因表达分析，发现大多数宿主转录物从 6 hpi 开始下调，并持续整个感染期，尽管在 12 至 72 hpi 阶段上调的 unigene 数量有所增加。值得注意的是，KEGG 注释中最丰富的途径是代谢途径，表明宿主幼虫代谢机制在 HvAV-3h 感染后受到高度影响。此外，在大多数情况下，宿主表皮蛋白编码 unigenes 高度下调，表明宿主幼虫表皮合成被病毒感染抑制。
