Shi Yanhua, Lin Weiping, Chu Jinxin, Wang Guohui, Zhao Punan, Huang Guo-Hua, Hou Dianhai
School of Life Science and Technology, Weifang Medical University, Weifang, 261053, People's Republic of China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Arch Virol. 2022 Apr;167(4):1075-1087. doi: 10.1007/s00705-022-05402-0. Epub 2022 Mar 5.
Analysis of orthology is important for understanding protein conservation, function, and phylogenomics. In this study, we performed a comprehensive analysis of gene orthology in the family Ascoviridae based on identification of 366 protein homologue groups and phylogenetic analysis of 34 non-single-copy proteins. Our findings revealed 90 newly annotated proteins, five newly identified core proteins for the family Ascoviridae, and 14 core proteins for the genus Ascovirus. A phylogenomic tree of 11 Ascoviridae members was constructed based on a concatenation of 35 of the 45 ortholog groups. In combination with phosphoproteomic results and conservation estimations, 30 conserved phosphorylation sites on 17 phosphoproteins were identified from a total of 176 phosphosites on 57 phosphoproteins from Heliothis virescens ascovirus 3h (HvAV-3h), providing potential research targets for investigating the role of these protein in the regulation of viral infection. This study will facilitate genome annotation and comparison of further Ascoviridae members as well as functional genomic investigations.
直系同源分析对于理解蛋白质保守性、功能和系统发育基因组学至关重要。在本研究中，我们基于366个蛋白质同源组的鉴定以及34个非单拷贝蛋白的系统发育分析，对正痘病毒科家族中的基因直系同源进行了全面分析。我们的研究结果揭示了90个新注释的蛋白质、5个新确定的正痘病毒科家族核心蛋白以及14个正痘病毒属核心蛋白。基于45个直系同源组中的35个串联构建了11个正痘病毒科成员的系统发育树。结合磷酸化蛋白质组学结果和保守性估计，从烟芽夜蛾痘病毒3h（HvAV - 3h）的57个磷酸化蛋白上总共176个磷酸化位点中，鉴定出17个磷酸化蛋白上的30个保守磷酸化位点，为研究这些蛋白在病毒感染调控中的作用提供了潜在的研究靶点。本研究将有助于进一步对正痘病毒科成员进行基因组注释和比较以及功能基因组学研究。
