State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China.
Bioresour Technol. 2018 Sep;263:562-568. doi: 10.1016/j.biortech.2018.04.120. Epub 2018 May 2.
One-pot bioconversion is an economically attractive biorefinery strategy to reduce enzyme consumption. Direct conversion of lignocellulosic biomass for butyric acid production is still challenging because of competition among microorganisms. In a consolidated hydrolysis/fermentation bioprocessing (CBP) the microbial structure may eventually prefer the production of caproic acid rather than butyric acid production. This paper presents a new bioaugmentation approach for high butyric acid production from rice straw. By dosing 0.03 g/L of Clostridium tyrobutyricum ATCC 25755 in the CBP, an increase of 226% higher butyric acid was yielded. The selectivity and concentration also increased to 60.7% and 18.05 g/L, respectively. DNA-sequencing confirmed the shift of bacterial community in the augmented CBP. Butyric acid producer was enriched in the bioaugmented bacterial community and the bacteria related to long chain acids production was degenerated. The findings may be useful in future research and process design to enhance productivity of desired bio-products.
一锅法生物转化是一种具有经济吸引力的生物炼制策略，可以降低酶的消耗。由于微生物之间的竞争，木质纤维素生物质的直接转化用于生产丁酸仍然具有挑战性。在整合的水解/发酵生物加工（CBP）中，微生物结构最终可能更倾向于生产己酸，而不是生产丁酸。本文提出了一种从稻草中生产高丁酸的新型生物强化方法。在 CBP 中添加 0.03g/L 的酪丁酸梭菌 ATCC 25755，丁酸产量增加了 226%。选择性和浓度也分别提高到 60.7%和 18.05g/L。DNA 测序证实了强化 CBP 中细菌群落的变化。丁酸产生菌在生物强化细菌群落中得到了富集，而与长链酸生产相关的细菌则退化了。这些发现可能对未来的研究和工艺设计有用，以提高所需生物产品的生产力。
