School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20850, USA.
Cell Chem Biol. 2018 Aug 16;25(8):941-951.e6. doi: 10.1016/j.chembiol.2018.04.012. Epub 2018 May 17.
Vitamin B is made by only certain prokaryotes yet is required by a number of eukaryotes such as mammals, fish, birds, worms, and Protista, including algae. There is still much to learn about how this nutrient is trafficked across the domains of life. Herein, we describe ways to make a number of different corrin analogs with fluorescent groups attached to the main tetrapyrrole-derived ring. A further range of analogs were also constructed by attaching similar fluorescent groups to the ribose ring of cobalamin, thereby generating a range of complete and incomplete corrinoids to follow uptake in bacteria, worms, and plants. By using these fluorescent derivatives we were able to demonstrate that Mycobacterium tuberculosis is able to acquire both cobyric acid and cobalamin analogs, that Caenorhabditis elegans takes up only the complete corrinoid, and that seedlings of higher plants such as Lepidium sativum are also able to transport B.
维生素 B 仅由某些原核生物合成，但许多真核生物如哺乳动物、鱼类、鸟类、蠕虫和原生生物（包括藻类）都需要它。关于这种营养物质如何在生命领域中运输，还有很多需要了解的地方。在此，我们描述了几种不同的钴胺素类似物的制备方法，这些类似物的主要四吡咯衍生环上连接有荧光基团。还通过将类似的荧光基团连接到钴胺素的核糖环上来构建进一步的类似物，从而生成一系列完整和不完整的钴胺素类物质，以跟踪在细菌、蠕虫和植物中的摄取。通过使用这些荧光衍生物，我们能够证明结核分枝杆菌能够获取 cobyrinic 酸和钴胺素类似物，秀丽隐杆线虫只摄取完整的钴胺素类物质，而高等植物的幼苗，如蕹菜，也能够运输 B。
